摘要
目的探讨天花粉蛋白对体内体外单纯疱疹病毒-1(HSV-1)的抑制作用。方法动物随机分为模型对照组和天花粉蛋白治疗组,小鼠颅内接种HSV-1建立病毒性脑炎模型,治疗组于接种HSV-1前30min腹腔注射天花粉蛋白注射液,接种病毒后12、24、48和96h、7d分别测定各组脑组织病毒滴定度。培养神经细胞分为正常对照组、病毒对照组、药物预处理组,接种病毒后12、24、48和96h分别测定各组培养神经细胞的病毒滴定度。结果天花粉蛋白治疗组于小鼠颅内接种HSV-1后12、24、48和96h、7d脑组织病毒滴度分别为(1.32±0.39)、(1.27±0.44)、(1.33±0.41)、(1.29±0.48)、(1.16±0.45),明显低于模型组(2.91±0.55)、(2.95±0.56)、(2.93±0.54)、(2.89±0.58)、(2.89±0.44),差异有显著性(P<0.001)。药物预处理组在12、24、48和96h后培养神经细胞病毒滴度分别为(1.12±0.34)、(1.18±0.40)、(1.24±0.38)、(1.26±0.42),明显低于病毒对照组(2.65±0.64)、(2.59±0.53)、(2.83±0.55)、(2.82±0.57),差异有显著性(P<0.001)。天花粉蛋白治疗组小鼠存活率为53.3%,模型组小鼠生存率为6.7%;天花粉蛋白预处理组神经细胞存活率均明显高于病毒对照组,差异有显著性(P<0.01)。结论天花粉蛋白对体内体外单纯疱疹病毒-1具有抑制作用。
[Objective]To evaluate the inhibitive effects of Trichosanthin （TCS） on Herpes simplex virus-1 （HSV-1） in vivo and vitro. [Methods] Eighty mice were randomly assigned into two groups： Model control group （model group, n =40）, TCS-treated group （treated group, n =40）. Viral encephalitis was induced by intracranial inoculation of HSV-1 in the two groups. The TCS-treated group was injected with TCS 30 minutes before HSV-1 inoculation. The viral titer was measured at 12, 24, 48 and 96 h, 7 d after HSV-1 inoculation, respectively. The culture neuron in vitro were assigned into three groups： Normal control group, HSV-1 control group, TCS-pretreated group. The viral Liter were measured at 12, 24, 48 and 96 h after HSV-1 were inoculated into the neuron, respectively. [Results] The viral titer of brain tissue at 12, 24, 48 and 96 h, 7 d after HSV-1 intracranial inoculation were markedly lower in the TCS-treated group （1.32±0.39）, （1.27±0.44）, （1.33±0.41）, （1.29±0.48）, （1.16±0.45） eompared with the Model group （2.91±0.55）, （2.95±0.56）, （2.93±0.54）, （2.89±0.58）, （2.89±0.44）, respectively （P 〈0.001）. The viral titer of neuron at 12, 24, 48 and 96h after HSV-1 inoeulation in the TCS- pretreated group （2.65±0.64）, （2.59± 0.53）, （2.83±0.55）, （2.82±0.57） were markedly lower than that in the HSV-1 control group （1.12±0.34）, （1.18±0.40）, （1.24±0.38）, （1.26±0.42）, respectively （P〈0.001）. The mice livability and the neuron livability in the TCS-treated group and TCS-pretreated group were significance higher than those of the Model group and HSV-1 control group, respectively （P 〈0.05）. [Conclusions] TCS has inhibitive effects on Herpes simplex virus-1 in vivo and vitro.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2008年第19期2760-2763,共4页
China Journal of Modern Medicine
基金
广东省医学科研基金资助项目(No:A2001629)
