摘要
以模式植物拟南芥为例,建立了一种克隆small RNA分子的技术平台,为今后开展small RNA分子的生物学功能研究提供技术支撑。通过用抗病信号分子水杨酸(SA)处理拟南芥叶片后,进行small RNA分子群体的分离与接头连接、PCR扩增、T-载体克隆与检测、测序分析和生物信息学分析等一系列实验,成功地克隆了一些small RNAs,并对其表达和功能进行了分析。
We have developed a reproducible method of small RNAs cloning from model plant Arabidopsis thaliana, which will facilitate the research on small RNAs in the future. We have successfully cloned several small RNAs from leaves of Arabidopsis treated by SA （signal molecule of disease resistance）, using isolation, adapter ligation, PCR amplification, T- clone, sequence and bio-information analysis and so on. Furthermore, we conducted analysis on their expression profiles and predicted functions of these small RNAs.
出处
《云南植物研究》
CSCD
北大核心
2008年第5期570-576,共7页
Acta Botanica Yunnanica
基金
云南省基金项目(2004C0051M)
国家自然科学基金重大项目(90408022)
中国高技术研究发展计划(863项目)(2006AA02Z129)
中国科学院"百人计划"择优资助项目
