摘要
目的探讨灯盏花素对大鼠糖尿病早期肾脏的保护作用及其对肾近球小管Na+,K+-ATPase活性的影响。方法将链脲佐菌素诱导的DM大鼠分为模型组(DM组)和灯盏花素治疗组(BR组),另设正常对照组(NC组)。BR组给予腹腔注射灯盏花素盐水注射液20mg(/kg·d),NC组和DM组大鼠给予同体积的生理盐水。4周后麻醉下行双侧输尿管插管,收集半小时尿并行心脏穿刺取血,测定尿量,检测血糖(BG)和血、尿肌酐,放免法(RIA)测定尿微量白蛋白、尿β2-微球蛋白及血清内源性洋地黄样物质,计算尿白蛋白排泄率和肌酐清除率。取双肾称重并计算肾脏肥大指数。在体视显微镜下手工分离单根PT,用液闪法测其Na+,K+-ATPase活性。结果DM组BG、尿量、肾脏肥大指数、尿β2-MG、UAER、Ccr及PT的Na+,K+-ATPase活性与NC组相比均显著性升高(P<0.01),而血清EDLS水平则显著性下降(P<0.01);灯盏花素治疗后,血清EDLS水平显著性升高(P<0.01),其他指标均明显下降(P<0.05)。结论灯盏花素对DM大鼠肾脏有明显的保护作用,其机制可能部分与下调PT的Na+,K+-ATPase活性有关。
Objective To investigate the renal protection of breviscapine （BR）and its effects on Na^＋,K^＋-ATPase activity in proximal tubule（PT） of early diabetic rats. Methods The diabetic rats induced by streptozotocin（STZ） were devided into model group（DM group） and BR treatment group（BR group）. Healthy rats served as normal control group（NC group）. The rats of BR group were intraperitoneal injected normal saline （NS） solution of BR 20mg/ （kg·d）, while rats of NC group and DM group were administered NS in the same way. Four weeks later, urine of rats was collected for half hours from two intra-ureteral cannulas under anesthesia and blood sample was obtained from the heart by puncture. The amount of urine was measured and blood glucose （BG）, creatinine of serum and urine were assayed. The microalbumin and β2-microglobulin （β2-MG） of urine and endogenous digitalis-like substance （EDLS） of serum were assayed by radioimmunoassay （RIA）. From the data above, the urine albumin excretion rate （UAER）, creatinine clearance（Ccr） were calculated. The two kidneys of rats were extracted and weighed and hypertrophy index were calculated. The single PT was microdissected manually and examined by scanning electronmicroscope. Na^＋,K^＋-ATPase activity of PT was determined by liquid scintillation counter using radiochemical assay. Results In DM group, BG, amount of urine,kidney hypertrophy index, urine β2-MG, UAER, Ccr and Na^＋, K^＋-ATPase activity in PT were significantly higher than that in NC group （P〈0.01）, the level of EDLS concentration in serum,however,was significantly decreased compared with the NC group （P〈0.01）. In BR group,compared with the DM group, although no difference in BG, the level of EDLS concentration in serum were increased significantly （P〈0.01）, while others were decreased significantly after BR therapy （P 〈 0.05 ）. Conclusion There is an obvious renal protective effect of BR in diabetic rats and the mechanism, at least pa
出处
《中国现代医生》
2008年第26期26-28,共3页
China Modern Doctor
基金
珠海市科技计划项目(编号:PC200310057)
