摘要
背景：LARS韧带由法国JP Laboureau 1985年设计并应用于临床。中短期临床应用报道证实该人工合成韧带疗效满意，但目前该韧带材料移植后组织学转归的研究较少。目的：应用聚对苯二甲酸乙二醇酯材料LARS韧带重建兔前交叉韧带，对移植后移植物组织学及超微结构进行观察。设计、时间及地点：随机对照动物实验，于2007—01／12在苏州大学附属第二医院实验中心及相关实验室完成。材料：聚对苯二甲酸乙二醇酯材料LARS韧带为手术中剩余的LARS韧带残端共3条（上海科隆有限公司提供）；健康成年新西兰白兔12只。方法：采用系统抽样法将兔分为两组，自体前交叉覆盖残端组（L-LARS）共9膝，右膝前交叉韧带完全切断后，应用体外编织的聚对苯二甲酸乙二醇酯材料LARS韧带重建，并应用前交叉韧带残端覆盖LARS韧带；单纯LARS韧带组（LARS）共3膝，前交叉韧带完全切除后单纯应用体外编织的聚对苯二甲酸乙二醇酯材料LARS韧带重建前交叉韧带。主要观察指标：术后1，3，6个月切取关节内、骨道内移植物及关节内滑膜组织行苏木精-伊红染色及Masson染色观察，并应用透射电子显微镜观察移植物术后6个月的超微结构。结果：术后1个月，L-LARS组关节内LARS韧带纤维束被纤维结缔组织包裹，而LARS组手术后6个月仍不能被纤维结缔组织包裹。术后3个月，L-LARS组关节内LARS韧带纤维束包裹的纤维结缔组织为不成熟的胶原纤维组织，L-LARS组和LARS组骨道内或LARS韧带纤维束之间，均存在中等程度的异物反应或炎症反应，以LARS组明显。术后6个月，L-LARS组关节内LARS韧带纤维束之间仍为不成熟的胶原纤维组织；L-LARS组及LARS组骨道内或LARS韧带纤维束之间，异物反应或炎症反应逐渐减轻；L-LARS组及LARS组骨道内均存在骨长入，新生编织骨起始于骨道壁，向人工材料的深层�
BACKGROUND： LARS ligament was designed by JP Laboureau in 1985. Clinical applications have proved the artificial ligament is satisfactory in a middle- and short-term, but few reports the histological turnover after implantation. OBJECTIVE： To study the histological characteristics and ultrastructure of polyethylene terephthalate （PET） LARS artificial ligament after the reconstruction of anterior cruciate ligament in rabbits. DESIGN, TIME AND SETTING： Randomized control experiments were conducted at the laboratory in the Second Affiliated Hospital of Soochow University from January to December in 2007. MATERIALS： PET LARS ligaments were the stump of artificial posterior cruciate ligament obtained from operations （provided by Shanghai Kelong Company Limited）. Twelve skeletally matured New Zealand white rabbits were used in this study. METHODS： Twelve rabbits were divided into two groups according to systematic sampling. The PET LARS was transplanted to substitute the original anterior cruciate ligament, and the transplanted PET LARS was covered with the remnant of anterior crnciate ligament in 9 rabbits （L-LARS group）, while only PET LARS was transplanted in 3 rabbits, which no covering with the remnant of anterior crnciate ligament （LARS group）. MAIN OUTCOME MEASURES： The grafts and synovinm were harvested at 1, 3 and 6 months after implantation, and were processed into the stain by hematoxylin-eosin and Masson. Transmission electron microscope investigation was performed on the grafts at 6 months to observe ultrastructural findings. RESULTS： At l month after implantation, the grafts in joints were covered with recipient connective tissues in L-LARS group, but were not covered anything after 6 months in LARS group. At 3 months, there were moderate to severe inflammatory reaction or foreign body reaction adjacent to the LARS fibers in bone tunnel or between LARS ligament fiber bundles. At 6 months, there were still irregularly aligned collagen fiber bundles slightly or in some portions
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第36期7061-7066,共6页
Journal of Clinical Rehabilitative Tissue Engineering Research
