摘要
目的了解德国小蠊的抗药性与磷酸酯酶和谷胱甘肽S-转移酶(GSTs)生化特性变化的关系,以初步揭示其抗性机制。方法参照Bessey等的方法测定磷酸酯酶活性与动力学参数Km和Vmax。参照Clark、Kao和Booth等的方法测定GSTs活性与动力学参数Km和Vmax。结果敏感品系与野生品系德国小蠊的酸性磷酸酯酶活力分别为0.98和26.95 nmol/(只.30 min),比活力分别为1.45和1.59μmol/(mg pro.30 min),Km值分别是26.14和0.89 mmol/L,Vmax值分别是3.33和0.85 nmol/(mg pro.30 min);敏感品系与野生品系德国小蠊的碱性磷酸酯酶活力为(0.03±0.00)和(0.28±0.06)nmol/(只.30 min),比活力分别为(0.33±0.00)和(0.37±0.00)μmol/(mg pro.30 min),Km值分别是70.38和61.24 mmol/L,Vmax值分别是16.20和14.00 nmol/(mg pro.30 min);敏感品系与野生品系德国小蠊GSTs活力分别是0.13和0.47 nmol/min,比活力分别为8.94和17.37 nmol/(mg pro.30 min);Km值分别是2.08和5.81 mmol/L,Vmax值分别是0.08和0.17 nmol/min。结论磷酸酯酶与GSTs在野外品系的抗性形成过程中起一定作用。
Objective To study the relationship between the activities of glutathione S-transferase （GSTs） and phosphatase from German cockroach（Blattella germanica ） and its resistance, so as to reveal its resistance mechanism. Methods The activity of phosphatase and the values of the Michaelis-Menten constant （Km ） and the maximum velocity （ Vmax ） were determined according to the method of Bessey et al. The activity of glutathione s-transferase and the values of its Km and Vmax were measured according to the methods of Clark, Kao and Booth. Results The activity of acidic phosphatase from sensitive strain and field strains were 0.98 and 26.95 nmol/（ insect·30 min）respectively, and the specific activities of them were 1.45 and 1.59 μmol/（mg pro·30 min）. The Km values were 26.14 and 0.89 mmol/L, and the values of the Vmax were 3.33 and 0.85 nmol/（mg pro·30 min） respectively. The activity of alkaline phosphatase （ALP） from two strains were （0.03 ± 0.00） and （0.28±0.06）nmol/ （insect·30min）, and their specific activities were （0.33±0.00） and （0.37±0.00）μmol/ （mg pro· 30 min） respectively. The values of the Km were 70.38 and 61.24 mmol/L, and the values of the Vmax were 16.20 and 14.00 nmol/（mg pro·30 min）. The activity of GSTs from two strains were 0.13 and 0.47 nmol/min respectively,and the specific activities were 8.94 and 17.37 nmol/（mg pro·min） respectively. The values of the Km were 2.08 and 5.81 mmol/L, and the values of the Vmax were 0.08 and 0.17 nmol/min. Conclusion The activity change of glutathione s-transferase and phosphatase from field strain German cockroaches was related to the resistance.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
北大核心
2008年第5期422-425,共4页
Chinese Journal of Vector Biology and Control
关键词
德国小蠊
磷酸酯酶
谷胱甘肽S-转移酶
抗药性
German cockroaches（Blattella gerrnanica ）
Phosphatase
Glutathione S-transferase
Resistance
