摘要
在利用乳酸菌Lactococcus lactis1.009细胞中的谷氨酸脱羧酶转化制备γ-氨基丁酸(GABA)的过程中,探讨了菌体浓度、缓冲液种类及浓度、转化时间、菌龄、磷酸吡哆醛添加量、底物添加方式等因素对GABA产量的影响。结果表明,细胞转化法制备GABA的最佳条件为:菌体浓度10g/L,菌龄14h,缓冲液为0.2mol/L pH4.7的醋酸缓冲液,PLP的添加量为0.1mmol/L。通过多次分批添加谷氨酸,50℃下反应60h后,GABA的积累量可达19.1g/L,转化率为99.9%。
γ-aminobutyric acid was prepared by L-Glutamate decarboxylase from Lactococcus lactis 1.009. The influence of cell concentration, buffer system,cell age, reaction time and external PLP concentration were studied. The optimum reaction system was 0.2moVL HAc-NaAc buffer pH4.7, containing 10g/L lactic acid bacteria and 0.1mmol/L pyridoxal phosphate （PLP）. Glutamate was added each 10h, after 60h GABA content was 19.1 g/L, the conversion of L-Glutamate was 99.9%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2008年第9期166-169,共4页
Science and Technology of Food Industry
基金
国家自然科学基金重点项目(20436020)
