摘要
Objective To examine the effect of deglycosylation on gating properties of rNav1.3. Methods rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control. Results Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV. Conclusion Glycosylation altered the gating properties of rNav 1.3 and contributed to the functional diversity.
目的观察糖基化对rNav1.3电压门控性质的影响。方法将rNav1.3在爪蟾卵母细胞中表达,衣霉素抑制其糖基化,双电极电压钳记录全细胞电流,Origin软件分析处理数据。对照组为未去糖基化的rNav1.3。结果与糖基化的rNav1.3相比,去糖基化的rNav1.3稳态激活曲线向去极化方向偏移约10mV,稳态失活曲线向超极化方向偏移约8mV。结论糖基化修饰改变rNav1.3的电压门控性质,异化其生理功能。
基金
the National Basic Research Development Program of China (No. 2006CB500801).
