摘要
目的研究胰岛素样生长因子-Ⅰ受体（IGF-IR）与胃癌发生发展的关系。方法应用免疫组化法检测40例手术切除胃癌组织及其远端正常胃黏膜中IGF-IR表达水平。Western印迹法检测胃癌细胞系SGC7901和MGCS03中IGF-IR的表达水平。设计并体外合成靶向IGF-IR的小干扰RNA，将其转染到MGC803细胞中，Western印迹法检测转染后48hIGF-IR蛋白表达水平，MTT法检测细胞增殖能力，细胞计数并描记生长曲线。结果胃癌组织IGF-IR表达阳性率为75．5％，显著高于远端正常胃黏膜（25％，P〈0．01）。IGF-IR表达与TNM分期和淋巴结转移相关（P〈0．05），但与性别、年龄、分化程度、肿瘤浸润深度无关（P〉0．05）。胃癌细胞株中有IGF-IR高表达，小干扰RNA1组IGF-IR表达抑制率可高达89．80％±4．10％，转染后第5天细胞增殖降至最低点（为空细胞组的29．0％±4．0%），同期细胞数降至对照组的21．15％士1．10％。结论IGF-IR在胃癌细胞中过表达，应用RNA干扰技术可有效沉默IGF-IR基因，抑制肿瘤细胞的增殖。
Objective To study the relationship of insulin-like growth factor- I receptor （IGF- IR） and carcinogenesis of gastric cancer. Methods The expression of IGF- IR was detected in 40 cases of resected gastric cancer tissues and adjacent normal gastric muensa by immunohistochemistry. The expression of IGF- IR in gastric cancer cell lines （ MGC803 and SGC7901） was detected by Western Blot. siRNAs targeted to IGF- IR were designed, synthesized and transfected into MGC803 cells, the changes of IGF- IR protein level were detected by Western Blot at 48 h after transfection, the cell proliferation was examined by MTT, and then the growth curve was obtained. Results The positive rate of IGF-IR expression in gastric cancer tissues was 75.5%, significantly higher than that of adjacent normal tissues （25%, P〈0.01）. The expression level of IGF-IR was related with TNM stage, lymphnode metastasis （P〈 0. 05）, but not related with sex, age, histological differentiation, invasion depth of gastric cancer （P〉0. 05）. Intense expression of IGF-IR was showed in gastric cancer cell lines. The inhibition ratio of IGF-IR expression in siRNA1 group were 89. 80% ±4. 10%, the cell proliferation decreased to minimum level at the fifth day after transfection （by 29.0 % ± 4.0 % of mock-treated group）, the cell number decreased bY 21.15 %± 1.10% of mock treated group at the same time. Condesions IGF-IR is over-expressed in gastric cancer cells and can be effectively silenced by RNA interferes, therefore the growth of tumor cell was inhibited. Thus, it indicates that IGF-IR may be a promising target for gene therapy of gastric cancer.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2008年第8期522-526,共5页
Chinese Journal of Digestion
关键词
胃肿瘤
胰岛素样生长因子-Ⅰ受体
RNA干扰
Gastric neoplasms Insulin-like growth factors- Ⅰ receptor
RNA interference
