摘要
焦磷酸测序是一种新型的DNA测序技术,近年来开始应用于单核苷酸多态性(SNPs)检测。组织蛋白酶基因(Cathepsin-L,CTSL)在对虾的蜕皮周期中起重要的作用。本研究利用焦磷酸测序技术,对96个凡纳滨对虾的CTSL基因序列(GenBank登录号:AY366355)的C681G SNP位点进行检测分型。结果发现C/C、C/G和G/G基因型,频率分别是0.81、0.16和0.03,频率分布符合Hardy-Weinberg平衡。统计分析结果表明在该群体中C681G SNP和体重关系不显著(P>0.05)。研究结果显示焦磷酸测序技术是一种高效的SNP检测技术,可以在对虾的遗传研究中发挥重要作用。
Pyrosequencing is a new DNA sequencing method, it was used to analyze single nucleotide polymorphisms recently years. Cathepsin-L gene is a lysosomal protease gene that is involved in the intermoh cycle of shrimp. In this study, genotyping of cathepsin-L gene （GenBank accession no. AY366355） SNP （C681G） in 96 Litopenaeus vannamei shrimps was performed using pyrosequencing. The C/C,C/G and G/G genotype were found in this population, and the frequency were 0.81, 0. 16 and 0.03, respectively. The frequency distribution was consistent with the law of Hardy-Weinberg. The statistic analyses showed that C681G SNP had no significantly association BW in this population （P〉0.05）. The results of this study showed that pyrosequencing is a versatile technique that could improve the efficiency of SNP analysis for shrimp genomic research.
出处
《水产学报》
CAS
CSCD
北大核心
2008年第5期684-689,共6页
Journal of Fisheries of China
基金
国家科技支撑计划项目(2006BADOIA13)
广西十百千人才专项基金(2007113)
关键词
凡纳滨对虾
焦磷酸测序
组织蛋白酶基因
单核苷酸多态性
Litopenaeus vannamei
pyrosequencing
cathepsin-L gene
single nucleotide polymorphisms （SNP）
