摘要
为了评价肺癌单克隆抗体检测良恶性胸腔积液的诊断与鉴别诊断价值，同步采集临床上明确诊断的６３例良恶性胸腔积液患者的胸水和血清，以及１０例正常人的血清，进行肺癌单抗金标免疫斑点渗透法的检测，并与癌胚抗原的检测比较。结果显示：恶性胸腔积液组肺癌单抗金标免疫斑点渗透法和癌胚抗原的阳性率明显高于良性胸腔积液组及正常组（Ｐ＜０．０１），而且肺癌单抗金标免疫斑点渗透法检测的阳性率高于癌胚抗原。在恶性胸腔积液中，肺癌单抗金标免疫斑点渗透法的灵敏度和特异度分别为６５．１％和１００．０％，癌胚抗原的灵敏度和特异度分别为５３．５％和９５．０％，两项指标联合检测的灵敏度和特异度分别为７６．７％和９５．０％。说明肺癌单抗金标免疫斑点渗透法对良恶性胸腔积液有鉴别诊断价值，而且优于癌胚抗原；两项指标联合检测可以提高对恶性胸腔积液的诊断符合率。
Benign and malignant pleural effusion was assayed with monoclonal antibodies of lung cancer to evaluate their diagnostic and differential diagnostic value. The pleural effusion and serum of 63 patients with the benign and malignant pleural effusion diagnosed clearly in clinic and 10 normal serum were tested by dot immunogold filtration assay of monoclonal antibodies of lung cancer (DIGFA). The assay was compared with carcinoembryonic antigen (CEA). The results showed that the positive rates of DIGFA and CEA were much higher in malignant pleural effusion group than in benign disease and normal group ( P <0.01), and higher in DIGFA than in CEA ( P >0.05). The sensitivity and specificity of DIGFA were 65.1% and 100.0%, of CEA 53.5% and 95.0%, of combined assay of two markers 76.7% and 95.0% respectively in the malignant pleural effusion. The conclusions suggest that DIGFA has differential diagnostic value in benign and malignant pleural effusion, and is superior to CEA. The combined assay of two markers can improve diagnostic accord rates in malignant pleural effusion.
出处
《实用癌症杂志》
1997年第3期178-180,共3页
The Practical Journal of Cancer
关键词
肺肿瘤
单克隆抗体
胸腔积液
诊断
Monoclonal antibodies Lung neoplasms Carcinoembryonic antigen Pleural effusion Diagnosis
