摘要
目的检测运动神经元病（ＭＮＤ）病人血清中是否存在运动神经元特异抗原成分，并探索ＭＮＤ潜在的诊断标志物。方法制备５株抗运动神经元单克隆抗体，并证明其对大鼠脊髓前角运动神经元具有高度特异的免疫组织化学反应。应用抗运动神经元单克隆抗体２４Ｂ０－ＭｃＡｂ，用ＥＬＩＳＡ法对２５例运动神经元病病人血清中的特异抗原成分进行检测。根据临床表现将２５例病人分为肌萎缩侧索硬化（ＡＬＳ）、脊肌萎缩症（ＳＭＡ）及进行性球麻痹（ＰＢＰ）３组，再按年龄段分３个亚组（＜２０岁组、２０～３９岁组、＞４０岁组）。结果发现８５％（２２／２５）临床确诊的ＭＮＤ病人存在较高浓度的特异抗原成分，ＭＮＤ病人与正常对照组对２４Ｂ０－ＭｃＡｂ的反应性差异有显著性意义（Ｐ＜０．０５），ＡＬＳ、ＳＭＡ及ＰＢＰ亚型之间差异也有显著性意义（Ｐ＜０．０５），而年龄组之间差异虽有显著性意义，其临床意义尚需进一步研究。性别组之间的差异无显著性意义。结论ＭＮＤ病人血清中存在运动神经元特异抗原成分。用抗运动神经元单克隆抗体以ＥＬＩＳＡ法检测运动神经元特异抗原可以作为诊断ＭＮＤ的辅助检查。
Objective To test whether the motor neuron specific antigen (MNSA) could be detected in motor neuron disease (MND) patient, and to exploit the potential diagnostic marker of MND. Methods Five monoclonal antibodies against motor neurons (McAbMn) were prepared, which exhibited highly specific immunohistochemical reaction with the ventral horn motor neurons of the rat spinal cords. The sera from the 24 patients with MND and the 10 controls were screened for MNSA using McAbMn with ELISA. The serums of the patients with MND were divided into three different groups, i.e., Amyotrophic lateral sclerosis (ALS), the spinal muscular atrophy (SMA), and the progressive bulbar palsy (PBP) group. They were then subdivided into 4 age groups (ages of 30￣39 years, 40￣49 years, 50￣59 years and 60￣69 years). Results 85% (22/24) of all the patients clinically diagnosed as MND showed higher serum MNSA levels compared with the control patients. A significant difference was found between the MND group and the control group (P<0.05). When the patient groups were examined in more details, no significance was showed in different age groups, but the serum MNSA levels were significantly different in the MND patients with ALS and with PBP. Conclusion MNSA existed in the MND patients. The MNSA might reflect the progressive accumulation on account of the deaths of the motor neurons in MND and that the ELISA of serum MNSA would be a helpful potential diagnostic test for MND.
出处
《中华神经科杂志》
CAS
CSCD
1997年第6期342-345,共4页
Chinese Journal of Neurology
基金
国家自然科学基金
