摘要
目的探讨吗啡对大鼠心肌急性缺血/再灌注损伤的保护效应是通过激活何种特异性阿片受体起作用的。方法健康SD大鼠共50只,雌雄各半,随机分成单纯缺血/再灌注组、吗啡组、吗啡+κ受体拮抗药组、吗啡+δ受体拮抗药组、假手术组,每组10只,建立大鼠心肌缺血/再灌注模型。分别于缺血后10min(mycardial ischenmic 10 minute,MI10min)及再灌注4.5h(myocardi-al ischemic-reperfusion 4.5 hours,MIR4.5h)采血及采取组织标本,采用放免法测定血浆肿瘤坏死因子α(TNF-α),常规测定心肌酶(CK、CK-MB),用红四氮唑(TTC)染色测定心肌梗死面积。结果吗啡组及吗啡+δ受体拮抗药组较其他实验组可显著降低大鼠CK、CK-MB(P<0.05),缩小心肌梗死面积(P<0.05)。结论证实吗啡是经由κ阿片受体对大鼠缺血/再灌注损伤心肌起保护作用的。
Objective It is to explore that what is the specific opioid receptor can produduee the eardioproteetive effect induced by morphine in acute ischemie - perfusion in rats. Methods Half part of healthy female and male Spargue - Dawley （ SD ） rats （ n = 50） were randomly divided into 5 groups （ 10 rats in each group） , ischemia/reperfusiongroup （I/R） group, morphine group, morphine ＋ Nor - BNI （ nor - Binahorphimine dihydrochloride, a selective κ - opioid receptor antagonist ） group, morphine ＋ NTI （ Nahrindole hydrochloride, a selective δ - opioid receptor antagonist ） group, and control group. The left coronary anterior descending branch（LAD） of rats were subjected to 10 minutes of occlusion and 4. 5 hours of reperfusion. Radioimmunoassay was used to detect tumor necrosis factor alpha （ TNF - α ） in serum. Routine method was used to detect creatine （CK） , creatine kinase （CK- MB） in serum. The rats heart were harvested for determination of myocardial infarct size by TTC on computer analysis system after 4. 5 hours of reperfusion. Results In morphine group, morphine ＋ NTI group, Plasma CK, CK - MB decreased markedly in MIR at 4. 5 h compared with in I/R group and morphine ＋ Nor - BNI group （ P 〈 0. 05 ）. Conclusion The study has shown that morphine can have a protective effect on ischemic - reperfusion in rats myocardium by exciting a selective opiate receptor.
出处
《中国急救医学》
CAS
CSCD
北大核心
2008年第9期819-821,共3页
Chinese Journal of Critical Care Medicine
基金
甘肃省科学事业费科研项目(No.QS061-C33-38)
关键词
吗啡
阿片受体
缺血/再灌注
心肌梗死面积
Morphine
Opioidreceptor
Ischemic - reperfusion
Myocardial infarct size
