摘要
以商品乙酰胆碱酯酶(来自家蝇)和自制的鸡肝乙酰胆碱酯酶、鲤鱼脑乙酰胆碱酯酶为研究对象,比较3种不同浓度的有机磷农药(氧乐果、敌百虫和敌敌畏)对这3种酶的酶活抑制率。结果表明,3种农药对家蝇乙酰胆碱酯酶的抑制率最高;对鸡肝和鲤鱼脑乙酰胆碱酯酶的酶活抑制率比家蝇低;3种乙酰胆碱酯酶对氧乐果都不敏感。在检测精度要求不高的情况下可用鲤鱼脑酶作为敌敌畏的快速检测用酶,用鸡肝酶作为氧乐果的快速检测用酶。家蝇AChE是快速检测敌百虫的敏感酶。
The commercial acetylcholinesterase （ACHE） from Musca domestica, and the acetylcholinesterase made by the researchers themselves from fowl liver and cyprinoids brain, were used for pesticides detection. The inhibition of AChE activity under different concentrations of omethoate, trichlorfon and dichlorvos were compared. The results show that the inhibition of AChE activity of Musca domestica is the highest of the three above. Three kinds of Acetylcholinesterase are not sensitive to omethoate. AChE from cyprinoids brain can be used for rapid detection of dichlorvos in the case of high detection precision is not needed. AChE from fowl liver can be used for rapid detection of trichlorfon.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2008年第9期83-86,共4页
Transactions of the Chinese Society for Agricultural Machinery
基金
山东省科技攻关计划资助项目(项目编号:2008GG10009027)
山东省教育厅科技计划资助项目(项目编号:J07YF11)
关键词
农药残留
检测
乙酰胆碱酯酶
抑制率
Pesticide residues, Detection, Acetylcholinesterase, Inhibition
