摘要
采用含不同植物叶片汁液的培养基对银杏(Ginkgo biloba)链格孢菌(Alternaria sp.)进行离体培养,并设定加糖和不加糖2组处理,对银杏链格孢菌生物学特性进行了初步研究。结果表明,2组试验菌丝生长速率与分生孢子产生的趋势基本一致:加糖处理的生长情况明显好于不加糖处理;而含玉米、水稻、豇豆、花生等植物叶片汁液的培养基生长情况较好,而以含银杏、丝瓜叶片汁液培养基生长情况较差。病菌菌丝体在散射光照射及黑暗条件下生长较快,直射光下生长较慢;分生孢子在缺少光照或黑暗条件下萌发率较高。菌丝体生长的适宜pH为5～8;分生孢子萌发的适宜pH为6～8。菌丝体生长的适宜温度为20～30℃;分生孢子萌发的适宜温度为25～30℃。菌丝体在相对湿度50%～100%范围内均可生长;相对湿度达90%以上时,分生孢子才能萌发。室内毒力测定表明,以烯唑醇对菌丝生长的抑制率最高,其毒力回归方程的相关系数及EC50值分别为0.985和0.17ai.μg/mL,多菌灵、福美双毒力中等。
The biological characteristics of Alternaria sp.from Ginkgo biloba’s leaf were studied.The media with different plant leaf juices were prepared and the treatments with or without sugar were divided respectively.The results showed that on the treatments with sugar,the colony grew obviously faster than those growing on the treatments without sugar.Among the treatments,the colony grew faster on the corn,rice,cowpea and peanut leaf juice medium than it did on both ginkgo and loofah leaf medium.The mycelia grew faster both in scattering rays and on the darkness than it did in direct rays,the germination rate of conidia on the darkness was higher than it was in both direct rays and scattering rays.The suitable pH for mycelia growth was 5～8.The suitable pH for conidia germination was 6～8.The suitable temperature for mycelia growth was 20～30 ℃.The suitable temperature for conidia germination was 25～30 ℃.The mycelia could grow with RH from 50% to 100%;only under high relative humidity(RH≥90%),could the conidia germinate.The toxicity of 3 kinds of fungicide to the Alternaria sp.showed that diniconazole was the best in all tested fungicide.The correlation coefficient and EC50 was 0.985 and 0.17 ai.μg/mL respectively.Both carbendazim and thiram belonged to middle toxicity.
出处
《长江大学学报（自科版）（中旬）》
CAS
2008年第3期1-4,共4页
Journal of Yangtze University(Nature Science Edition)
