摘要
通过化学麻醉剂丁香油、MS-222对施氏鲟(Acipenser schrenckii)幼鱼[(71.2±8.9g)]进行了急性毒性试验和麻醉试验,同时研究了丁香油、MS-222和直流电对施氏鲟幼鱼的麻醉效果,并在行为学基础上探讨了以上三种麻醉方法对鲟的有效性。毒性试验结果表明:丁香油和MS-222对施氏鲟幼鱼的24、48、72、96h的LC50分别为26.37mg/L、22.17mg/L、18.00mg/L、16.22mg/L和61.67mg/L、58.22mg/L、56.90mg/L、56.90mg/L。MS-222的毒性明显小于丁香油,MS-222的安全浓度为15.57mg/L,丁香油的安全浓度为4.70mg/L。麻醉试验结果显示:鱼体在3min内失去平衡,7min内完全麻醉,并可在3min内恢复的化学麻醉浓度最低为MS-222100mg/L,丁香油50mg/L、直流电电压12V。麻醉时间与麻醉剂浓度和直流电电压均呈幂函数相关,相关系数R2均大于0.9。在适宜的直流电压(24V)下,不同水温之间的麻醉效果无显著差异;在适宜的麻醉浓度(MS-222为100mg/L,丁香油为50mg/L)下,麻醉所需要的时间与水温呈直线负相关,而恢复时间与水温呈直线正相关,相关系数R2均大于0.9。以上结果表明,三种方法均适用于麻醉鲟,应根据不同目的选用适当的麻醉方法。其中电麻醉具有麻醉时间和恢复时间极短的优点,值得进一步探讨其生理麻醉机制。
Experiments were conducted to anaesthetic effect of MS-222, clove oil and investigate the acute toxicity of MS-222 and clove oil, and the direct current on juvenile Acipenser schrenckii[（71.2±8.9 g）], Besides, the validity of the three anaesthetic methods of the sturgeon on the ground of behavior observation was discussed. The result of toxicity experiments show that the 24 h L50, 48 h L50, 72 h L50, and 96 h L50 of clove oil and MS-222 to juvenile Acipenser schrenckii were 26.37 mg/L, 22.17 mg/L, 18.00 mg/L, 16.22 mg/L and 61.67 mg/L, 58.22 mg/L, 56.90 mg/L, 56. 90 mg/L, respectively. The toxicity of MS-222 was obviously lower than clove oil, the safe concentration of clove oil and MS-222 were 4.70 mg/L and 15.57 mg/L respectively. The result of the anaesthesia experiments showed that： the fish lost its equilibrium within 3 minutes, and was completely at narcosis anaesthesia in 7 minutes. When exposed to a high concentration （MS-222 above 100 mg/L, clove oil above 50 mg/L, direct current above 12 V）, and the fish recovered in 3 minutes. It was found that the relationship between the time, anaesthetic concentration and the direct current followed an exponential function, the related coefficient R^2 was more than 0.9. The difference of the anesthetic results under different water temperature with the suitable direct current （24 V） was not significantly different. Under suitable anesthetics concentration （MS-222 100 mg/L, clove oil 50 mg/L）, the complete anaesthesia time and water temperature were negative correlation, while recovered time and water temperature were positive correlation, and the related coefficient R2 were all more than 0.9. All the results indicated that three methods were all applicable to anaesthetize sturgeon, but the proper method should be chosen according to the different purposes. The anaesthesia time and recovery time in the electricity anaesthesia were so short that its physiology mechanism deserved further research.
出处
《上海水产大学学报》
CSCD
北大核心
2008年第5期539-546,共8页
Journal of Shanghai Fisheries University
基金
国家“八六三”计划项目(2004AA603110)
国家自然科学基金重大项目(30490234)
国家“十五”科技攻关项目(2004BA526B0114)
中央级公益性科研院所基本科研业务费专项资金项目(2007M02)
上海市长江口中华鲟自然保护区科研专项
关键词
施氏鲟
直流电
MS-222
丁香油
温度
急性毒性
安全浓度
Acipenser schrenckii
direct current
MS-222
clove oil
temperature
acute toxicity
safety concentration
