摘要
采用分步法SDS-PAGE和改良A-PAGE分析了49份小麦农家品种等资源的Glu-1,Glu-3,Gli-1位点基因组成.结果表明,Glu-1位点编码的HMW-GS组成共检测到14种图谱,其中占比例最大图谱(N,7+8,2+12)达44.9%,其次是(N,7+9,2+12)和(1,7+8,2+12)各占14.3%,(1,7+9,5+10)和(2*,7+9,2+12)各占4.1%,其余9种图谱各占2.0%;Glu-3位点编码的LMW-GS组成共检测到25种图谱,(a,j,c)占12.2%,(a,g,c)占10.2%,(new,g,c)占8.2%,(a,g,a),(a,j,a),(f,g,c)和(d,j,a)各占6.1%,(f,j,a),(d,g,c),(a,f,c)和(f,f,c)各占4.1%,其余14种图谱各占2.0%,Glu-A3位点新发现的基因图谱占14.3%;Gli-1位点编码的醇溶蛋白组成共检测到40种图谱,(a,f,l)和(f,l,i)各占6.1%,(f,l,a),(new,c,i),(b,g,l)和(l,l,a)各占4.1%,其它34种图谱各占2.0%,Gli-1位点新发现的基因图谱占16.3%;扬白麦、迟亚草、葫芦头麦、红秃头、红粒白芒、红火麦、矮孟牛IV中在Glu-3或Gli-1位点含有新发现的等位基因.
The banding patterns of HMWgs, LMWgs and HMW gliadins of 49 wheat landracs were analyzed using two-step SDS-PAGE and a modified A-PAGE,respectively checked out according to the 20 standard cuhivars＇ patterns. 14 HWMgs band patterns were encoded by Glu-1 locus,the pattern with the highest frequency was （ N,7 ＋ 8,2 ＋ 12） （44.9%）, next came （ N,7 ＋ 9,2 ＋ 12 ） and （ 1,7 ＋ 8,2 ＋ 12 ）, taking up 14.3 %, respectively, （ 1, 7 ＋ 9,5 ＋ 1 0 ） and （2 ° , 7 ＋ 9,2 ＋ 12 ）, taking up 4.1%, respectively and the rest 9 patterns took up 8.2%. A total of 25 LMWgs band patterns were encoded by Glu-3 locus and the pattern with the highest frequency was （a,j, c） taking up （12.2%） , next came （a,g,c） （taking up 10.2% ） ,（new,g,c） （taking up 8.2% ） ,（a,g,a） ,（a,j,a）,（f,g,c） ,（d,j,a） , （ taking up 6.1% ） , （ f, j, a） , （ d, g, c ） , （ a, f, c ） , （ f, f, c ） （ taking up 4.1% ） , more than 5 alleles were at Glu-A locus and 16.3% of them were newly discovered, 6 alleles were at GIu-B 1 locus,3 alleles were at Glu-D 1 locus ; 40 HMW gliadin band patterns were encoded by Gli-1 locus,more than 7 alleles were at Gli-A 1 locus,and 12.2% of them were newly discovered, 10 alleles were at Gli-B 1 locus,7 alleles were at Gli-D 1 locus;There are 8 landraces such as Yangbaimai,Chiyacao,Huloutumai,Hongtutou, Honglibaimai, Xiaohongmang, Honghuomai, Aimengniu IV which has new alleles at Glu-3 or Gli-1 loci.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2008年第4期385-390,共6页
Journal of Henan Agricultural University
基金
陕西省科技攻关项目(2003-k01-05)
西北农林科技大学育种专项(05Y2004-2)
