摘要
从梅花鹿鹿茸中提取了天然鹿茸总多肽（VATP）.在进一步分离纯化的过程中,筛选出具有促进成骨细胞增殖的高活性肽组分（VAP-B）,并通过制备型HPLC对其纯化,得到了分子量分布约为200～600的小肽活性组分（VAP-B2）.细胞周期分析结果表明,鹿茸肽VAP-B2组分促进了人成骨肉瘤细胞OS-732的周期转化,使其细胞周期S期细胞指数明显增加,即表现为S期DNA含量明显提高.碱性磷酸酶（ALP）活性检测结果表明,随着鹿茸肽VAP-B2剂量的增加,ALP的活性明显增加.这与成骨细胞增殖分化及成熟过程中,细胞的周期性变化和骨形成标志物碱性磷酸酶水平的明显增高相符.
The native total peptides （VATP）were extracted from velvet antlers of sika deer （Cerv us nippon Temminck）. A high active peptide component （VAP-B） stimulating proliferation of a human osteosarcoma cells was isolated from VATP. Finally, a further purified active peptide component（ VAP-B2 ） with molecular weight distribution from about 200-600 was obtained from VAP-B by preparative HPLC. VAP-B2 was able to enhance the average content of DNA in S phase. And also the alkali phosphatase （ALP） activity in osteosarcoma cells increased obviously with the increase of VAP-B2 dosage and treatment time. The results are consistent with cell cycle variation and ALP level as a marker of bond formation during osteocyte proliferation and differentiation, as well as maturity. The results obtained in this study provide a clue to explore a new peptide medicine from velvet antlers for therapy of osteoporosis.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2008年第9期1791-1796,共6页
Chemical Journal of Chinese Universities
基金
国家中医药管理局科学基金(批准号:2004ZDZX003)
吉林省科学技术厅科研基金(批准号:200705288)资助
关键词
梅花鹿鹿茸
鹿茸肽
分离与纯化
生理活性
成骨细胞
Velvet anders of sika deer
Sika deer peptides
Isolation and purification
Physiologic activity
Osteosarcoma cells
