摘要
目的探讨促肝再生磷酸酶3（PRL-3）蛋白在结直肠癌（CRC）组织中的表达及其与肿瘤浸润、转移等病理学特征的关系，以及其对预后的判断价值。方法采用免疫组织化学方法检测46例CRC组织及其对应正常黏膜、6例结直肠腺瘤（CRA）及其对应正常黏膜、29例转移淋巴结及6例肝转移灶中的PRL-3表达情况，分析其表达与临床病理学指标的关系；根据随访结果建立生存曲线．分析PRL-3表达与预后的关系。结果PRL-3在结直肠正常黏膜和CRA中几乎无表达：46例CRC组织中有26例（56．5％）阳性表达；29例转移淋巴结中有26例（89．7％）阳性表达：6例肝转移灶中有5例阳性表达。PRL-3在CRC细胞浆中呈弥漫性表达，而在细胞膜上表达更集中和强烈。PRL-3表达与肿瘤分期（P=0．019）和淋巴结转移（P=0．026）密切相关，而与患者年龄、性别、肿瘤分化程度、大小和位置无关（P〉0．05）。CRC中有淋巴结转移的29例（63．0％）PRL-3阳性率高于未发生淋巴结转移的17例（37．0％）（P=0．001）。中位随访时间为41．4个月，CRC表达PRL-3蛋白阴性的患者累计生存率显著高于阳性表达者（P=0．032）。结论PRL-3可以作为预测CRC患者预后的潜在生物学指标。
Objective To investigate the expression of phosphatase of regeneration liver-3 （PRL-3） protein and its relationship with tumor invasion and metastasis in human eolorectal carcinoma, and elucidate prognostic value. Methods Immunohistochemistry method was applied to detect the PRL- 3 expression in the primary tumor specimens and paired paratumor normal tissues from 46 colorectal carcinoma patients, the adenoma tissues from 6 patients with colorectal adenoma, all the metastastie lymph nodes from 29 cases and the metastastic liver lesions from 6 cases. The relationship between PRL-3 expression and clinicopathologic parameters was analyzed and a survival curve was achieved according to Kaplan-Meier method. Results No or weak PRL-3 protein expression was detected in normal colorectal mucosa and colorectal adenoma. In colorectal carcinoma tissues, PRL-3 expression was confirmed in 26 of 46 cases （56.5%） of primary, coloreetal carcinomas （with lymph node metastasis 63.0%, without lymph node metastasis 37.0%, P=0.001）, 26 of 29 （89.7%） lymph node metastases, and 5 of 6 liver metastases. The expression of PRL-3 was assembled in the cytoplasm of carcinoma cells and more intensively on the cell membrane.Analysis of the relationship between PRL-3 expression and the clinicopathologic features showed that PRL-3 expression was closely associated with tumor stage （P=0.019）, lymph node metastasis （P=0.026）, but no relationship with age, sex, tumor size, degree of differentiation was founded （P〈0.05）. The mean follow-up time was 41.4 months and results showed that patients with positive expression of PRL-3 had a significantly poorer prognosis than those with negative PRL-3 expression group （P=0.032）. Conclusions PRL-3 protein plays a novel role in tumor progression and metastasis of colorectal careinoma. PRL-3 ean be expeeted to be a potential predictive biomarker for identifying the prognosis in eolorectal carcinoma patients.
出处
《中华胃肠外科杂志》
CAS
2008年第5期487-491,共5页
Chinese Journal of Gastrointestinal Surgery
关键词
结直肠肿瘤
肿瘤转移
促肝再生磷酸酶3
预后
Coloreetal neoplasm
Tumor metastasis
Phosphatase of regeneration liver-3
Prognosis
