摘要
背景:近年来对肝细胞生长因子的研究已证实肝细胞生长因子具有促进血管生长,减轻胃黏膜损伤的作用。目的:观察以减毒沙门氏菌为载体携带肝细胞生长因子基因对束缚-水浸应激诱发大鼠应激性胃溃疡模型的影响。设计、时间及地点:随机区组设计,实验于2007-03/09在解放军兰州军区兰州总医院和甘肃省中医学院进行。材料:40只雄性Wistar大鼠随机分为4组:正常组、模型组、预防组、对照组,每组10只。方法:应激前用1×1012cfu/L携带肝细胞生长因子基因的减毒沙门氏菌给预防组大鼠灌胃,隔天一次,共灌胃6次,每只大鼠0.3mL/次,同时用同剂量单纯减毒沙门氏菌同方法给对照组大鼠灌胃,正常组与模型组用等体积的碳酸氢钠(10%NaHCO3)同法灌胃。将模型组、预防组和对照组大鼠用束缚-水浸应激法复制应激性胃溃疡模型,18h后,麻醉后将4组大鼠处死。主要观察指标:采用比色法和放射免疫分析法检测大鼠血清一氧化氮和血清皮质醇含量,大体及光镜下观察大鼠胃黏膜病理改变,测定胃溃疡指数。结果:40只大鼠均进入结果分析。①模型组大鼠胃黏膜出现明显的出血及糜烂,预防组的黏膜损伤明显轻于模型组和对照组,应激后预防组的溃疡指数明显低于模型组和对照组(P<0.01)。②模型组血清一氧化氮含量明显高于正常组(P<0.01),预防组血清一氧化氮的含量则明显低于模型组(P<0.01)。③模型组血清皮质醇含量明显高于正常组(P<0.01),预防组血清皮质醇的含量虽高于正常组但明显低于模型组(P<0.01)。结论:携带肝细胞生长因子基因的减毒沙门氏菌可通过减少血清中的一氧化氮和皮质醇的含量,预防应激性胃溃疡的发生。
BACKGROUND： In recent years, hepatocyte growth factor （HGF） has been proved to promote the growth of vessels and relieve gastric mucosal injury. OBJECTIVE： To explore the protective effect ofHGF gene mediated by attenuated Salmonella on the gastric ulcer model induced by water immersion and restraint stress in rats. DESIGN, TIME AND SETTING： Randomized experiments were carried out in the Lanzhou General Hospital, Lanzhou Military Area Command of Chinese PLA and Gansu College of Traditional Chinese Medicine （Lanzhou, Gansu, Province） from March to September in 2007. MATERIALS： Forty Wistar male rats were randomly divided into four groups： normal group, model group, prevention group and control group, with ten rats in each group. METHODS： Before stressing, rats in the prevention group and in the control group were treated by intragastric administration of 1 ×10^12 cfu/L attenuated Salmonella carrying HGF and attenuated Salmonella, respectively. The dose administrated was 0.3 mL for each animal, once another day, 6 times together. Same volume of 10% NaHCO3 was administrated to the model and normal control rats. The stress-induced gastric ulcer model of rats in the model group, prevention group and control group were established by water immersion and restraint stress. All rats were killed 18 hours later. MAIN OUTCOME MEASURES： The concentration of nitrogen monoxide and cortisol in rat serum was detected by chromatometry and radioimmunity, respectively. The pathological changes were observed grossly and under light microscope. Ulcer index was also evaluated. RESULTS： Forty rats were all involved in the result analysis. Severe mucosal bleeding and erosion were observed in rats from the model group. But in rats from the prevention group, the mucosal injury changes were evidently ameliorated compared to the model group and control group. The ulcer index of rats in the prevention group was significantly lower than that of rats in the control group and the model group （P 〈 0.01）. The level o
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第37期7286-7289,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
甘肃省科技厅支撑计划项目(200701)~~
