摘要
目的:建立一种快速、准确检测单纯疱疹病毒Ⅱ型D蛋白基因的方法。方法:根据已发表的gD蛋白核苷酸序列设计引物,采取降落PCR对gD蛋白DNA进行扩增。结果:从2份病毒株中均扩增出与预期大小完全一致的目的片段,并且采用降落PCR能有效地降低或避免非特异性扩增。结论:TD-PCR能够用于快速,准确地检测gD蛋白,为生殖器疱疹的鉴别诊断在分子水平上提供了理想的新方法。
Objective：To establish a method which can test instantly and precisely the protein D gene of herpes simplex virus type Ⅱ.Methods：After designing a primer according to the published protein D nucleotide sequence,the DNA of protein D was amplified with the touchdown PCR（TD-PCR）.Results：The objective segments were amplified from two herpes simplex virus type Ⅱ strains whose sizes were exactly the same as expected,and it could reduce or avoid the non-specific amplification effectively.Conclusion：The TD-PCR can be used for instant and precise test of protein D.It provides a new and ideal method for the genital herpes diagnosis at the level of molecule.
出处
《中国卫生检验杂志》
CAS
2008年第9期1735-1736,共2页
Chinese Journal of Health Laboratory Technology
基金
杭州市科技发展计划项目(20080333Q29)
