摘要
目的：建立分析女贞子中主要裂环环烯醚萜苷类成分女贞苷（nuezhenoside）及女贞苷G13（G13）的HPLC定量分析方法，并考察了不同采集时间及不同产地女贞子样品中上述2种成分的含量。方法：以国产YMG-C18（4．6mm×250mm，10μm）为色谱柱，流动相为乙腈-水，梯度洗脱（乙腈：0min，20％；15min，25％），流速为1mL·min^-1，检测波长240nm，室温（25℃）。结果：女贞苷和女贞苷G13分别在0．341～34．1μg，0．331—33．1μg与峰面积线性良好；平均回收率分别为97．5％，RSD1．4％（n=3）和98．1％，RSD1．8％（n=3）。不同地区女贞子药材中女贞苷和女贞苷G13的含量差别较大，可能与不同品种来源及加工炮制方法等因素有关；实验数据表明，采集期在11月初至中旬上述2种成分的含量最高。结论：所建立的HPLC梯度洗脱法可用于同时测定女贞子药材中主要裂环环烯醚萜苷类成分女贞苷及女贞苷G13的含量，可作为女贞子中水溶性指标性成分的检测方法，为全面控制女贞子药材内在质量提供参考。
Objective： To develop an HPLC method for determination of two major secoiridoid glucosides （nuezhenoside and G13） from Fructus Ligustri Lucidi. and determinae nuezhenoside and G13 in samples from different acquisition times and different places. Method： The sample was separated on a YMG-C18（4. 6 mm ×250 mm, 10μm） column. The mobile phase was acetonitrilewater, gradient elution （CH3CN;0 min, 20%; 15 rain, 25% ） with the flow rate of 1 mL·min-1, the detection wavelength was 240 nm, and the column temperature was set at 25 ℃ Result： In HPLC analysis ,the linear ranges of nuezhenoside and G13 were 0. 341- 34. 1μg and 0. 331-33.1μg, respectively; their average recoveries were 97. 5% （RSD 1.35% ） and 98.1% （RSD 1.82% ）, respectively. The results of content determination （nuezhenoside and G13） of samples from different places varied greatly. This may be caused by different species sources and different preparation methods, etc. The experiment led up to the fact that from the beginning to the middle of November the content of nuezhenoside and G13 reached the maximum. Conclusion： The established method can be used to determine two major secoiridoid glucosides （nuezhenoside and G13） in Fructus Ligustri Lucidi simultaneously. Meanwhile it can be used for the quality control of Fructus Ligustri Lucidi.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第18期2099-2102,共4页
China Journal of Chinese Materia Medica
