摘要
目的探讨转化生长因子-β1(TGF-β1)和碱性成纤维生长因子(bFGF)蛋白在儿童肾小球疾病中的表达及其与儿童原发性肾小球疾病的相关性。方法采用免疫组化方法(Envision二步法)检测微小病变(MCNS)10例、系膜增生性肾小球肾炎(MsPGN)25例、局灶节段硬化(FSGS)8例、膜性肾病(MN)7例,观察TGF-β1、bFGF蛋白在儿童肾小球疾病的表达情况。结果在不同肾小球疾病患儿肾小球上皮细胞及肾小管间质细胞均有不同程度的TGF-β1和bFGF蛋白的阳性表达,与正常肾组织比较差异有统计学意义(P<0.001)。小管间质TGF-β1和bFGF的蛋白表达明显高于肾小球内表达,差异有统计学意义(P<0.05)。随肾小管间质损伤和炎症细胞浸润程度及纤维化程度加重,TGF-β1和bFGF蛋白表达明显增加(P<0.05)。TGF-β1、bFGF的表达以FSGS最强,其次依次为MN、MsPGN、MCNS。同步观察发现TGF-β1与bFGF肾内表达在阳性染色强度及分布上基本一致,二者之间存在显著正相关。肾小球疾病肾组织TGF-β1、bFGF表达水平与尿RBP、24h尿蛋白定量显著相关(P<0.05)。结论TGF-β1与bFGF在肾组织内的共同表达,提示二者对肾小球疾病的进展可能有协同作用。其过度共表达参与了肾小球疾病的进展,主要参与肾小管-间质的损害。因此,阻断TGF-β1和bFGF信号传导,可能会延缓肾小球硬化的进展。
Objectives To explore the expressions of transforming growth factor-β1（TGF-β1）and basic fibroblast growth factor（bFGF）in the kidneys of children with glomerulonephropathy,and to explore the possible mechanisms of glomerulonephropathy. Methods Fifty patients were enrolled in the study. Among them,10 patients were diagnosed with MCNS,25 with MsPGN,8 with FSGS, and 7 with MN. The expressions of TGF-β1,bFGF were evaluated with immunohistochemical techniques. Results The expressions of TGF-β1 and bFGF proteins were increased in glomerular cells and tubule interstitial cells in patients with various glomerular diseases. The increase was significant in the patients compared with normal renal tissues（P 〈 0.001）. The expressions of TGF-β1 and bFGF were higher in the tubule interstitial tissues than in glomerulus（P 〈 0.05）. The expressions of TGF-β1 and bFGF increased with the severity of the tubule interstitial damage, infiltration of inflammatory cells and fibrosis of the renal tissues. The gradation of increased expressions of TGF-β1 and bFGF were FSGS 〉 MN 〉 MsPGN 〉 MCNS. The intensity of the expression and distribution of TGF-β1 and bFGF were basically similar,suggesting that a positive correlation between these two factors. The expressions of TGF-β1 and bFGF in the renal tissues correlated with the urine RBP,24 hours urine quantitative protein measurement（P 〈 0.05）. Conclusions Increased expressions of TGF-β1 and bFGF might suggest that these two factors play important roles in the development of renal tubule and interstitial fibrosis in pediatric glomerulonephropathy. Arrestment of the signal conduction of the TGF-β1 and bFGF may retard the evolution of the glomerulosclerosis.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2008年第9期788-791,共4页
Journal of Clinical Pediatrics
关键词
儿童
肾小球疾病
转化生长因子-Β1
碱性成纤维生长因子
肾小管间质损伤
肾纤维化
免疫组化
children
glomerulonephropathy
transforming growth factor-β1
basic fibroblast growth factor
renal tubule and interstitial fibrosis
immunohistochemical techniques
