摘要
目的检测我国不同地区动物中狂犬病毒带毒率并分析糖蛋白编码基因序列。方法ELISA、免疫荧光法分别检测586份采集自中国不同地区的犬、猫、蝙蝠和野鼠脑标本和16份犬唾液中狂犬病街毒，阳性标本乳鼠颅内接种，并测序。结果ELISA、免疫荧光法均在犬脑中分离到10株狂犬病街毒，其中贵州省113份犬脑中分离到2株病毒，湖南省62份犬脑中分离到2株病毒，武汉市70份犬脑中分离到2株病毒，江苏省85份犬脑中分离到4株病毒，沈阳市69份犬脑中未分离到狂犬病毒；79份猫脑、100份蝙蝠脑及8份鼠脑中未检出狂犬病街毒，16份犬唾液标本未检出狂犬病毒。在贵州省和武汉市，冬季采集的112份犬脑未检出狂犬病毒，春夏季采集的40份犬脑中，4份阳性。分离的10株狂犬病毒阳性株颅内接种乳鼠后，均发病死亡。所有分离株均属狂犬病毒基因1型，可分为4个亚组。结论同一地区的狂犬病毒分离株以及相邻省份的狂犬病毒分离株的同源性十分接近，动物样本采集时间与狂犬病毒阳检率有关。
Objective To detect the positive rate of rabies virus in animals of different regions in China and to analyze the sequences of glycoprotein encoding gene. Methods Rabies street viruses were detected in 586 brain samples of dogs, cats, bats, mice and 16 saliva samples of dogs from different regions in China using enzyme-linked immunosorbent assay （ELISA） and immunofluorescent assay （IFA）. Positive samples were inoculated intracranially to neonate rats and then sequenced. Results Ten street virus strains were isolated from dog brains using ELISA and IFA, in which 2 positive of 113 in Guizhou Province, 2 positive of 62 in Hunan Province, 2 positive of 70 in Wuhan city, 4 positive of 85 in Jiangsu Province, and no positive of 69 in Shenyang city. There was no detectable rabies virus in brains of 79 cats, 100 bats, 8 mice and in 16 saliva samples. One hundred and twelve brain samples from dogs collected in winter were all negative and 4 were positive of 40 samples collected in spring and summer in Guizhou Province and Wuhan city. The neonate rats all died of rabies after inoculated with isolated street virus strains. All the 10 isolates were genotype 1 of Lyssavirus and could be divided into 4 subgroups. Conclusions The rabies virus isolates are highly homologous in the same region and the adjacent provinces. The positive rate of rabies virus isolation in animals is related to the time of sample collection.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2008年第8期463-466,共4页
Chinese Journal of Infectious Diseases
基金
国家十五攻关课题资助项目（2004BA718803）
