摘要
目的探讨动物体内植入生物材料后机体免疫状态的改变。方法将生物材料聚乙烯醇水凝胶(PVA)/纳米羟基磷灰石(n-HA)+聚酰胺66(PA66)植于大鼠皮下,分别于术后第2、4、6周行局部组织学切片观察,检测大鼠血液中CD3、CD4+、CD8+含量以及脾细胞中IL-1、IL-6、TNF-α的含量并与术前对比。结果大鼠血液中CD3、CD4+、CD8+的含量和脾细胞中IL-1,IL-6,TNF-α的含量在第2、4、6周时与正常组对照差异无统计学意义。组织学切片显示血管和纤维组织进入复合材料网孔中,与复合材料连为一体,未产生排斥反应。结论PVA/n-HA+PA66生物材料植入动物体内后未引起动物机体的免疫排斥反应,未干扰机体的免疫系统,是一种无毒、易被机体耐受的生物材料。
Objective To observe immunoreaction to PVA/n-HA＋PA66 biological composite material after being implanted into animal body. Methods PVA/n-HA＋PA66 composite materials were implanted into mouse subcutaneous tissue. Histologial examination was performed at 2,4,6 weeks, the amount of CD3, CD4^＋, CD8^＋ in blood and IL-I,IL-6,TNF-α in spleen cells was measured at the same time. Results The amount of CD3, CD4^＋ ,CD8^＋ in mouse blood and IL-1 ,IL-6,TNF-α in mouse spleen cells were not different with the control groups at 2,4,6 weeks（P〉0.05）. The fibrous tissue and some blood vessels were found growing into the porous materials, which resulted in composite materials intergration of proliferative structure. The reject reaction was not found. Conclusion PVA/n-HA＋PA66 biological composite material does not cause immune rejection after being implanted into animal body, verifying its good biocompatibility.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2008年第5期728-731,共4页
Journal of Sichuan University(Medical Sciences)
