摘要
目的研究赖诺普利对糖尿病大鼠早期周围神经病变的防治作用。方法链脲佐菌素（STZ）诱导糖尿病，给药8周，观察对糖尿病大鼠周围神经功能及结构的影响；并测定神经组织超氧化物歧化酶（SOD）、丙二醛（MDA）、Na^＋K^＋-ATP酶活性，坐骨神经内膜毛细血管密度。结果赖诺普利改善神经组织的功能和结构；改善神经组织的氧化应激状态、提高Na^＋K^＋-ATP酶活性、促进神经内膜血管新生。结论赖诺普利是防治糖尿病周围神经病变的有效措施。
Objective To investigate the effects of lisinopril, an angiotensin-converting enzyme inhibitor, on diabetic peripheral neuropathy （DNP）. Methods Twenty-five Wistar rats underwent intravenous injection of streptozocin to establish diabetes models and 10 rats were injected with sodium citrate solution as normal controls. The diabetic rats were randomly divided into 2 groups ： lisinopril group treated with gastric perfusion of lisinopril daily for 8 weeks, and diabetic control group. The diabetic controls and normal controls were treated with gastric perfusion of water. Sciatic nerve electrode penetration method was used to measure the motor nerve conduction velocity （MNCV） and sensory nerve conduction velocity （SNCV）. Light and heat pain measuring apparatus was used to measure the pain threshold. Then the sciatic nerves were isolated. Electron microscopy was used to observe the ultra-structure. The contents of superoxide dismatase （SOD） and malonyldialdehyde （MDA）, and Na ^＋K ^＋ -ATPase activity were detected by chemical colorimetry. Immunohistochemistry was used to detect the CD34 in the sciatic nerve. The capillary density of sciatic nerve was calculated. Results The MNCV and SNCV levels of the lisinopril group were both lower than those of the 2 control groups （ all P 〈 0. 01 ）. The potency of heat pain leg retraction response of the lisinopril group was significantly shorter than that pf the diabetic control group （ P 〈 0. 05 ）. The pathological changes of the lisinopril group were milder than those of the other groups. The SOD level and the Na ^＋ K ^＋- ATPase activity of the diabetic group were significantly lower than those of the normal control group, and the MDA of the diabetic group was significantly higher than those of the other 2 groups （ all P 〈 0. 05 ）. And the SOD level and Na ^＋K ^＋-ATPase activity of the lisinopril group were significantly higher than those of the diabetic control group, and the MDA level of the lisinopril group was significantly
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第35期2513-2515,共3页
National Medical Journal of China
关键词
赖诺普利
糖尿病
周围神经系统病变
Lisinopril
Diabetes
Peripheral nervous system diseases
