摘要
采用高效液相色谱法研究了复方新诺明在大菱鲆体内的残留消除规律,探讨了饲料中添加中草药对其残留消除的影响。健康大菱鲆随机分成2组,对照组和试验组连续5d投喂复方新诺明药饵,从第6d起对照组投喂不含药物的空白饵料,试验组投喂中药三黄散药饵,连续投喂1个月。定期取样,测定复方新诺明残留情况。结果表明,在(10±2)℃水温下,两种主要成分磺胺甲基异噁唑和甲氧苄啶在大菱鲆血清、肌肉、肝脏中的消除半衰期如下:对照组为T1/2β血清7.86d和9.30d、T1/2β肌肉8.42d和17.46d、T1/2β肝脏10.93d和20.63d;试验组为T1/2β血清7.54d和8.76d、T1/2β肌肉7.42d和16.11d、T1/2β肝脏10.40d和18.63d。以可食性组织肌肉为残留靶组织,磺胺甲基异噁唑和甲氧苄啶消除到最高残留限量(0.01μg·mL-1和0.05μg·mL-1)以下,对照组需要61d和41d,试验组需要46d和26d,表明中药三黄散对复方新诺明在大菱鲆体内的消除具有一定的促进作用。
The tissues distribution, residue depletion of compound sulfamethoxazole and the influence of Chinese herbal medicine were investigated during multiple dosing in turbot at （10±2）℃ seawater. The healthy turbots were fed a medicated diet containing 2 500 mg·kg^-1 compound sulfamethoxazole, twice daily for 5 days. Then the turbots were divided into two groups with different experimental diets. The control group was fed a non-medicated diet, and the test group was fed a medicated diet containing 3 000 mg·kg^-1 Chinese herbal medicine for one month. Serum, muscle and liver were sampled at different times and analyzed for sulfamethoxazole （SMZ） and trimethoprin （TMP） by high-performance liquid chromatography （HPLC）. The results showed that the residual time of TMP in turbot was longer than SMZ. In serum, muscle and liver, the elimination half-lives of SMZ and TMP in the control group were 7.86 d and 9.30 d, 8.42 d and 17.46 d, 10.93. d and 20.63 d, and in the test group were 7.54 d and 8.76 d, 7.42 d and 16.11 d, 10.40 d and 18.63 d, respectively. The withdrawal period of SMZ and TMP were 61 d and 41 d in the control group, and 46 d and 26 d in the test group, respectively, when the concentrations in muscle fell below the maximum residue limits （MRLs） of 0.01 μg·mL^-1 and 0.05 μg·mL^-1. These results indicated that Chinese herbal medicine could promote elimination of compound sulfamethoxazole in turbot.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2008年第5期2096-2100,共5页
Journal of Agro-Environment Science
基金
公益性农业行业科研专项(nyhyzx07-046)
科研院所社会公益研究专项(2004DIB4J165)
关键词
复方新诺明
大菱鲆
中草药
残留
compound sulfamethoxazole
turbot （Scophthatmus maximus）
Chinese herbal medicine
residue
