摘要
目的建立寒热痹胶囊中芍药苷的含量测定方法。方法采用高效液相色谱法.色谱柱为Lichrospher ODS柱(4.6×250mm.5μm)分析柱。流动相为乙腈-0.1%磷酸溶液(13:87),检测波长为230nm.流速为1.0mL·min^-1柱温为30℃。结果本法精密度高,稳定性好,芍药苷进样量在0.137~0.684μg范围内具有良好的线性关系(r=0.9997),平均加样回收率为99.80%.RSD=1.43%(n=9)。结论本方法简便,准确,重现性好,可作为该制剂的含量测定方法。
OBJECTIVE To establish a method for determination of paeoniflorin in Hanrebi capsules. METHODS Using a HPLC method with Lichrospber ODS(4.6×250mm, 5μm) column, the mobile phase was acetonitrile-0.1% phosphoric acid (13:87), the detection wavelength was set at 230nm, flow rate was 1.0 mL·min^-1, and the column temperature was 30℃. RESULTS The precision and stability were fine, the standard curve of paeoniflorin was rectilinear within the range of 0.137 -- 0. 684μg, r = 0. 9997. The average recovery of paeoniflorin was 99.80% with RSD of 1.43% .CONCLUSION The method was simple, accurate and reproducible, and could be used for the determination.
出处
《海峡药学》
2008年第8期37-39,共3页
Strait Pharmaceutical Journal