摘要
目的建立消痔散的质量标准。方法采用TLC法对处方中的防风、甘草、花椒进行定性鉴别：采用高效液相色谱法洲定黄连中的盐酸小菜碱含量。色谱条件：Diamonsil C18（4．6mm×250mm．5μm）；乙腈：0．02mol·L^-1磷酸二氩钾溶液（32：68）为流动相；检测波长为265nm，流速1．OmL·min^-1。结果在TLC色谱中均能检出防风、甘草、花椒；盐酸小檗碱在0．36～1．08μg范围内呈良好的线性关系，r=O．9997，平均回收率为97．65％，RSD为1．2％。结论方法简便快速、结果准确。可有效控制消痔散的质量。
OBJECTIVE To establish the quality standards for Xiaozhi powder. METHODS Radix Saposh- nioviae, Radix Et Rhizoma Glycyrrhizae, Zanthoxylum bungeanum Maxim were identified by TLC, and the content of berberine hydrochloride was determined by HPLC. The chromatographic conditions included： Diamonsil Cls （4.6mm×250mm, 5μm）, acetonitrile-0.02mol·L^-1 potassium dihydrogen piaosphate（32： 68） was used as mobile phase, the UV detection wavelength was set at 265nm, the flow rate was 1.0mL·min^-1. The column temperature was 30℃. RESULTS Radix Saposhnioviae, Radix Et Rhizoma Glycyrrhizae, Zanthoxylum bungeanum Maxim could be identified by TLC. Berberine hydrochloride showed a good linear relationship at the range of 0.36 1.08ttg, r = 0. 9997. The average recovery was 97.65 %, and RSD was 1.2 %. CONCLUSION The method is accurate and can be used for the quality control of Xiaozhi powder.
出处
《海峡药学》
2008年第8期6-8,共3页
Strait Pharmaceutical Journal
