摘要
【目的】探讨丙戊酸钠(VPA)对慢性粒细胞白血病细胞株(K562细胞)增殖的影响及其可能机制。【方法】利用CCK-8法检测药物对细胞生长的影响;利用流式细胞仪检测药物处理后的细胞凋亡和细胞周期情况。【结果】VPA对K562细胞生长具有浓度-时间依赖性抑制作用;同时引起细胞凋亡增加(11.47%±0.25%),与正常对照组(4.77%±0.40%)比差异有统计学意义(P<0.05);G0/G1期细胞增多(82.30%±9.41%),S期细胞减少(12.88%±6.99%),细胞被阻滞在在G0/G1期(P<0.05)。【结论】VPA能够阻滞K562细胞于G0/G1期,最终抑制肿瘤细胞增殖并诱导其凋亡。
[Objective] To investigate the effects of sodium valproate （VPA） on the proliferation of chronic myeloid leukemia cell line K562,and to explore possible mechanisms. [ Methods ] K562 cells were treated with VPA. Cell proliferation was determined by CCK-8 assay. Cell apoptosis and cell cycle were analyzed by flow cytometry （FCM）. [Results] VPA inhibited the proliferation of K562 cells in concentration- and timedependent manners. The apoptosis rate was significantly higher in the cells treated with VPA than in untreated cells[ （11.47%±0.25%）vs （4.77%±0.40%）, P 〈 0.05]. After treatment of VPA, cell cycle was arrested obviously at G0/G1 phase （P 〈 0.05）. [Conclusions] VPA could induce G0/G1 phase arrest, inhibit the proliferation and induce the apoptosis of K562 cells in vitro.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2008年第5期562-565,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省医学科学技术基金(A2006193)
