摘要
以黄早四×齐319回交群体(BC2F1)为试验材料,在花期进行高强度干旱胁迫和耐旱导入系筛选,获得花期耐旱性显著高于亲本材料的玉米耐旱导入系。利用分子标记对其导入片段进行分析结果表明,在全基因组范围内,耐旱群体在36.59%的位点上含有供体亲本的特异标记,尤其在第4、5染色体上分别达到63.94%、56%,显著高于群体平均值;同时发现,导入频率的提高主要集中于染色体的部分区段,其中部分基因组区域与已定位的耐旱性相关QTL相邻或重叠。
A introgression lines （ILs） population for drought tolerance at flowering stage was developed by screening under highly intensive drought stress, from BC2F1 derived from the cross between maize inbred lines Huangzaosi and Qi 319. The results showed that the method could significantly improve the drought tolerance of the ILs compared with both parents. Genetic analysis showed that in chromosome 4 and 5, 63.94% and 56% of the loci possessed the donor allele, respectively, while the average was 36.59% for the whole genome. Introgressed segments were found to be located in a few specific genomic regions, some of which were previously proved to be related to drought tolerance. The results indicated that this method of developing ILs is valuable for mapping of drought tolerance related chromosome segments and developing of NILs of these genetic regions.
出处
《植物遗传资源学报》
CAS
CSCD
2008年第3期293-296,共4页
Journal of Plant Genetic Resources
基金
国家科技支撑计划项目(2006BAD13B03)
国家自然科学基金项目(30571133)
北京市自然科学基金重点项目(6071003)
关键词
玉米
耐旱
导入系
QTL
Maize
Drought tolerance
Introgession lines
QTL
