摘要
利用荧光标记法在反相高效液相色谱上对蚕蛹体内痕量前列腺素的分离、鉴定进行了研究，并对不同蛹期蛹体中前列腺素的组成和分布进行了测定。７种前列腺素（ＰＧＡ１，ＰＧＡ２，ＰＧＢ２，ＰＧＥ１，ＰＧＥ２，ＰＧＦ１α，ＰＧＦ２α）在所选择的实验条件下都能得到很好的分离，色谱柱为Ｃ８键合柱（φ６．０ ｍｍ ×１５０ ｍｍ），流动相为甲醇／水＝７０／３０，流速为１．５ ｍＬ／ｍｉｎ，荧光试剂为４－溴甲基－７－甲氧基香豆素；荧光检测波长为Ｅｘ＝３２５ｎｍ，Ｅｍ＝３９７ｎｍ，最小检测限为 １０－１０ｇ／ｍＬ．
The determination of prostaglandins (PG) in the extract of chrysalis was studied by reversed phase high performance liquid chromatography with fluorescence labeling. The results showed the variation of PG with the life time of chrysalis. The seven kinds of fluores- cence labeled prostaglandins (PGA1, PGA2, PGB2, PGE1, PGE2, PGF1α,PGF２α) could be separated and analyzed with a RP-C8 column (150 mm × 6 mm i. d. ) and methanol/water (70/30 V/V) as mobile phase at a flow rate of 1. 5 mL/min. The 4-bromomethyl-7- methoxycoumarin was used as the fluorescent reagent and the detection wavelength was Ex =325 urn and Em=397 urn. The minimum detectable level was 10-10g/mL.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1997年第12期1369-1373,共5页
Chinese Journal of Analytical Chemistry
关键词
蚕蛹
前列腺素
高效液相色谱
家蚕
Chrysalis, prostaglandin, fluorescence labeling, high performance liquid chromatography
