摘要
目的观察润肺散结胶囊(RSC)对Lewis肺癌(荷瘤)小鼠的抑瘤作用。方法采用动物移植性肿瘤实验法腋下接种Lewis肺癌制备小鼠荷瘤模型。将制模后小鼠随机分为模型对照组(给予生理盐水)、5-氟尿嘧啶注射液(5-Fu)对照组(15 mg/kg,每周6 d)及RSC 1.5、3.0和6.0 g·kg^(-1)·d^(-1)治疗组,每组10只。灌胃相应药物21 d后处死动物,观察瘤重、抑瘤率及瘤组织病理学变化。用免疫组化法检测瘤组织中Bcl-2和Bax蛋白的表达,以流式细胞仪分析脾细胞内T淋巴细胞的比例。结果RSC 1.5、3.0和6.0 g·kg^(-1)·d^(-1)组的抑瘤率分别为30.1%、35.0%和36.5%;与模型对照组比较,RSC各剂量治疗组瘤重和肿瘤体积均显著减小,差异均有统计学意义(P均<0.01)。瘤组织苏木素-伊红(HE)染色切片可见明显的炎细胞浸润和出血带,并有大片坏死区域,间质血管较少;电镜下见凋亡小体出现。与模型对照组比较,各用药组Bcl-2蛋白表达明显减少、染色变弱,吸光度值均显著减小(P均<0.01);Bax蛋白表达强度差异无统计学意义;RSC各剂量组CD3^+、CD4^+和CD8^+ T细胞比例均明显增高(P均<0.01)。结论润肺散结胶囊对体外培养的Lewis肺癌细胞有明显的抑制作用,其机制可能为诱导肿瘤细胞凋亡和提高机体免疫功能,为临床用药提供了理论依据。
Objective To investigate the mechanism and inhibitory effect of Runfei Sanjie capsule （RSC,润肺散结胶囊） on Lewis pulmonary tumor in mice. Methods Lewis lung tumor cultured in vitro was transplanted into subaxillas of mice to establish animal models with transplanted tumor. After the establishment of the models, the mice were randomly divided into model control group （only normal saline was given）, 5-fluorouracil （5-FU） control group （15 mg/kg, 6 days in every week） and RSC 1.5, 3.0, 6.0 g · kg^-1 · d^-1 treatment groups （each n= 10）. After the saline or the drug solutions were administered by gavage for 21 days into the corresponding groups, the changes of tumor weight, the rates of tumor inhibition and the pathological changes of tumor tissue were observed. The protein expressions of Bcl-2 and Bax in tumor tissue were detected by immunohistochemical method, and the proportion of T lymphocytes in splenocytes was analyzed by flow cytometry. Results The rates of tumor inhibition in RSC 1.5, 3.0, 6.0 g · kg^-1 · d^-1groups were 30.1%, 35.0% and 36.5% respectively; compared with the model control group, the quality of the tumor was more improved and the tumor mass was smaller in volume in the various RSC dosage groups, the differences being statistically significant （all P〈0.01）. The pathological examinations of hematoxylin and eosin （HE） sections of the tumor showed that there were marked infiltration of inflammatory cells, hemorrhagic bands, large areas of necrosis and less interstitial blood vessels; under the electron microscope, cell apoptosis was seen. Compared with the model control group, the protein expression of Bcl-2 was less obviously, staining weaker and the light absorbance value smaller markedly in various treatment groups （all P〈 0.01）, but in the comparisons of the intensity of protein expression of Bax among the groups, there was no significant difference. The proportions of T cells of CD3^＋, CD4^＋ and CD8^＋ were also greater in number in variou
出处
《中国中西医结合急救杂志》
CAS
2008年第5期289-292,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
广西壮族自治区医药卫生科研基金(Z2005191)
关键词
抗肿瘤
肺癌
流式细胞仪
凋亡
润肺散结胶囊
anti-tumor
lung cancer
flow cytometry
apoptosis
Runfei Sanjie capsule
