摘要
目的：研究原癌基因在哮喘发病中的作用．方法：以卵蛋白致敏诱发豚鼠哮喘建立实验模型．用分子杂交技术（Ｄｏｔ－ｂｌｏｔ、Ｎｏｒｔｈｅｒｎ－ｂｌｏｔ）分别观察正常及哮喘发作后ｃ－ｍｙｃ在豚鼠气道及肺组织中的表达水平．结果：原癌基因ｃ－ｍｙｃ在豚鼠哮喘发作后即刻表达增强，且呈时间依赖关系，并可被糖皮质激素部分抑制．结论：提示ｃ－ｍｙｃ在哮喘发病过程中起一定作用．
Objective: To study the roles of proto oncogene in asthma. Methods: The ovalbumin was used to induce expression of proto oncogene c myc in asthmatic guinea pigs. Using technique of molecular hybridization (Dot blot, Northern blot), the expression of c myc in normal and asthmatic pigs was observed. Results: There was a baseline mRNA expression of c myc in normal airway and lung tissue of the ginea pigs. Compared with the control, the c myc gene expression was increased much significantly and was time dependent. It reached a maximum at 30 min after inhaling ovalbumin. Whereas the inducement could be inhibited partly by dexamethasone. Conclusion: The results show that the increased oncogene c myc expression can be related to the attack of asthma.
出处
《第四军医大学学报》
1997年第5期401-403,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金
