摘要
目的:探讨使用巢式PCR检测孕妇外周血浆中男性胎儿DNA的可能性并评估其在早期判定胎儿性别方面的准确性。方法:采取20例正常孕妇外周血(孕10～38周)各2mL,枸橼酸钠抗凝后提取血浆,经蛋白酶K及十二烷基磺酸钠消化后直接作为模板,以巢式PCR对其中的胎儿DYS19基因位点进行检测,并将实验结果与娩出后的新生儿实际性别比较。结果:20例孕妇中有8例经2轮PCR后扩增出DYS19基因,后均证实为男胎;未扩增出DYS19基因的12例中有10例证实为女性,2例证实为男性。男胎性别预测符合率为80.0%(8/10)。结论:应用巢式PCR能够直接从母体外周血中检测到男性胎儿基因,且预测胎儿性别有较高的准确度。
Aim ： To investigate the possibility of finding male fetal DNA from maternal blood plasma with nested PCR method, and to evaluate its accuracy in fetal sex determination in early pregnancy stages. Methods：Peripheral blood was taken from each of the 20 pregnant women with pregnancy time ranging from 10 to 38 weeks, which were all DYS19 negative. Sodium citrate was used as anticoagulant. Plasma was extracted after centrifugation, SDS and proteinase K were added to digest plasma proteins, and the supernatant was used directly as the template of nested PCR to detect the DYS19 locus from fetuses. The experimental results were compared with the newborn＇s actual sex after delivery. Results ： Among all the 20 pregnant women, 8 of them were found to have DYS19 amplification bands after two rounds of PCR amplification, and were later confirmed to conceive male fetuses; 12 of them were found no DYS19 bands, among which 10 were confirmed to have female fetuses and 2 male fetuses. Prediction accuracy of male fetus was 80.0% （8/10）. Conclusion： The proposed method can directly detect male fetal genetic substances from maternal peripheral blood plasma, and the accuracy of male fetuses prediction is high.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2008年第5期940-943,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
郑州大学人才引进基金资助项目
