摘要
研究高胆固醇血症兔的血管内皮功能的异常状况及四氢生物喋呤(BH4)的转复机制。方法:18只新西兰兔,随机分为正常饮食组、高胆固醇饮食组、BH4加高胆固醇饮食组。喂养5周后,测定血中总胆固醇、一氧化氮(NO)及丙二醛水平,并取主动脉,进行离体血管舒张功能实验,测定不同条件下,血管条对乙酰胆碱的舒张反应。结果:与正常饮食组比较各胆固醇喂养组血清总胆固醇明显增高,有极显著性差异(P<0.01)。高胆固醇饮食组与正常饮食组比较血中NO水平明显减低(P<0.01),丙二醛浓度明显升高(P<0.01);BH4加高胆固醇饮食组与高胆固醇饮食组比较NO水平明显升高(P<0.05),而丙二醛的水平较高胆固醇饮食组明显降低。不同组别的动脉对乙酰胆碱最大舒张功能存在明显差别,高胆固醇饮食组的舒张功能较其他组明显下降;BH4加高胆固醇饮食组的舒张功能,较高胆固醇组明显恢复。结论:高胆固醇血症导致血管内皮细胞舒张功能障碍,BH4对其具有转复作用,其转复机制可能与BH4升高血液中的NO和降低过氧化物的水平有关。
Objective： To study the vascular endothelial cell dysfunctions in hypercholesteremia as well as the recovery mechanism of tetrahydrobiopterin in rabbits. Methods： Eighteen rabbits were randomized to receive normal diet （group A, n=6） , cholesterol diet （group B, n = 6）, cholesterol diet plus tetmhydropterin （group C, n=6） forSweeks. After plasma total cholesterol （TC）, triglycerides （TG）, No and malonaldehyde concentration was measured, thoracic aortas were removed and cut into ring segments about 5 mm long, then the endotheliurn-dependent maximal relaxation to Ach was obtained. Results： Compared with the control group, TC concentration in group B, C were markedly increased （p〈0. 01）. There was no difference among group B and C. Compared with group A, NO concentration in group B, C was markedly decreased, and malonaldehyde concentration was increased. Compared with group B, NO concentration in C group was markedly increased, but malonaldehyde concentration was decreased. Compared with group A, the maximal relaxations of arterioles induced by Ach were markedly decreased in group B and C. Compared with Group B, the maximal relaxations of arterioles induced by Ach were markedly increased in group C. Conclusion： Hypercholesterolemia results in the endothelial cell dysfunction. Tetrahydrobiopterin can restore endothelial Function in rabbits with hypercholesterolemia. This restoring is related to the increasing of NO concentration and the decreasing of malonaldehyde concentration.
出处
《四川生理科学杂志》
2008年第3期106-108,共3页
Sichuan Journal of Physiological Sciences
