摘要
目的 :观察 IFN- γ激活的巨噬细胞 ( MΦ)合成的一氧化氮 ( NO)及其在疟疾保护性免疫中杀伤疟原虫的作用。方法 :体外分离培养小鼠腹腔 MΦ,与不同浓度的 γ干扰素 ( IFN- γ)共育 4 8h后 ,加入分离的寄生疟原虫的红细胞 ,观察激活的 MΦ NO产生水平及其对疟原虫的杀伤作用。结果 :IFN-γ能激活 MΦ产生 NO,其产生的量与 IFN-γ的浓度有关 ;MΦ对疟原虫的杀伤作用与 NO的释放量呈显著相关关系 ( r=0 .898,n=13,P<0 .0 1) ;N-硝基 - L -精氨酸 ( N- nitro-L- arginine,L- NNA)能抑制 NO合成 ,同时也降低 MΦ 的杀伤活性 ;感染约氏疟原虫的 BAL B/c小鼠注射 L- NNA,导致原虫血症上升 ,小鼠死亡率升高。结论 :IFN- γ激活 MΦ产生 NO可能是杀伤疟原虫的重要作用机制。
AIM:To explore the role of nitric oxide (NO) produced by IFN- γ- activated macrophages in the killing of malaria parasite.METHODS:After the isolated murine peri- tonal macrophages were inoculated with IFN- γ of different concentrations for48h,P.yoelii- parasitized erytherocytes(PRBCs) were added for another4h.For in vivo studies mice were infected ip with1 0 7PRBCs,NO produced by IFN- γ- activated- macrophages both in vitro and in vivo were measured and the cytotoxicity of macrophage on Plasmodium yoelii parasites was examinded.RESUL TS:A positive correlation was found between the cytotoxicity of macrophages and NO levels(r=0 .898,P<0 .0 1 ) .L- NNA,an inducible NO synthase(i N- OS) competitive inhibitor,could inhibit the production of NO by macrophages and reduced the cytotoxicity of macrophages cytotoxicity on P.yoelii.IFN-γ was found to protect BALB/ c mice against P.yoelii infection in vivo,while injection of L- NNA resulted in high level of parasitemia and increased mortality in infected mice.CONCLUSION:The plasmodicidal cy- totoxicity could be attributed to the nitric oxide produced by the IFN- γ- activated macrophages.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1997年第6期335-339,共5页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
巨噬细胞
一氧化氮
约氏疟原虫
原虫血症
干扰素
Interferon- γ,macrophage s cytotoxicity,nitric oxide,Plasmodium yoelii,parasitemi
