摘要
以邻苯二甲酰亚胺为原料,经硝化、还原、巯基化和酰肼化反应合成6-氨基-7-巯基苯二甲酰肼(AMHP).AMHP分别与4-甲酰基苯甲醛、4-甲氧基苯甲醛和3,5-二溴-4-羟基苯甲醛制备不同的芳基苯并噻唑类发光试剂.对此类发光试剂的光谱行为进行研究,结果表明,芳基苯并噻唑衍生物是一类优良的发光试剂,在碱性条件下,这些试剂与铁氰化钾反应产生强烈的化学发光现象.它们中多数试剂的发光强度明显高于传统试剂鲁米诺,尤其以DB-p-H-AMHP发光强度超过鲁米诺的230倍为最佳,展现出良好的分析应用前景.
Phthalimide as raw material orderly reacted as nitration （HNO3/H2SO4）, reduction （SnCl2）, sulfhydryl reaction （NaSCN/Br2）, hydrazide reaction （NH2NH2/CH3CH2OH） respectively, sythesis 6-amino-7-mercapto- 2,3-dihydro-1,4-phthalazinedione （AMHP）. AMHP reacted with p-fomylbenzaldehyde, p-methoxylbenzaldehyde and 3,5-dibromo-4-hydroxylbenzaldehyde to make different compounds containing aromatics benzothiazole moiety which were abbreviated as p-C-AMHP, p-MO-AMHP and DB-p-H-AMHP, respectively. Then, the spectrum properties of new compounds were investigated in detail. The results show that their chemiluminescence intensities are obviously better than that of conventional illuminant luminol. Among these, DB-p-H-AMHP is the best and can produce chemiluminescence 230 times more intensely than luminal, which indicates that the reagent can be widely applied in analytical chemistry.
出处
《江南大学学报（自然科学版）》
CAS
2008年第4期488-492,共5页
Joural of Jiangnan University (Natural Science Edition)
基金
国家自然科学基金项目(20676052)
关键词
芳基苯并噻唑类试剂
化学发光
合成
aromatics benzothiazole moiety
chemiluminescence
synthesis
