摘要
目的了解磨牙牙根发育模式基因1（molar root patterning gene 1，Mrp1）的组织表达特征并对其结构进行预测、分析，从而为进一步研究磨牙牙根发育模式基因1的功能奠定基础。方法通过反转录聚合酶链反应（RT-PCR）方法对Mrp1基因的表达情况进行研究，并通过染色体分析、基因组定位及其他生物信息学手段对该基因进行结构分析。结果经RT-PCR检测发现，Mrp1 mRNA除了在大鼠磨牙牙胚组织表达外，在发育中的胰、肝、肺、肾组织中也有表达；生物信息学分析显示，Mrp1定位于大鼠18号染色体长臂18q12．3区，且Mrp1蛋白序列同尿道上皮细胞分化标志膜蛋白Uroplakin Ⅲb（p35）有37％的同源性，跨膜区分析及序列疏水性分析都印证了Mrp1蛋白质序列的第10～40位氨基酸间有一段跨膜序列及疏水区域。结论Mrp1基因编码产物可能是C端带有多个磷酸化位点的跨膜蛋白，且在多个脏器的发育中可能具有一定的生物学意义，值得进一步研究。
Objective To examine the expression of molar root patterning gene 1 （ Mrp1 ） and predict the Mrp1 structure by bioinformatics analysis. Methods A pair of Mrp1-specific PCR primers were designed, and RT-PCR method was used to study the mRNA＇s expression pattern in rat molar root and other organs. Gene positioning and other protein sequence prediction were carried out by chromosome analysis and other bioinformatios analysis. Results Mrp1 was expressed not only in the molar but also in the developing pancreas, liver, lung and kidney tissues. Mrp1 was located in the 18q12. 3 chromosome of the rats and the Mrp1 amino acids sequence had about 37 % homology with a known protein Uroplakin Ⅲ b （p35） which was an urothelial differentiation membrane molecular marker. A trans-membrane structure, 5 PKC phosphorylation sites and 4 CK Ⅱ phosphorylation sites in Mrp1 were found. Conclusions Mrp1 has a broad expression in different developing organs, and it may have a important function in the rat tooth root development.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2008年第9期528-530,共3页
Chinese Journal of Stomatology
关键词
基因
发育
逆转录聚合酶链反应
计算生物学
磨牙牙根发育模式基因1
Genes, developmental
Reverse transcriptase polymerase chain reaction
Computational biology
Molar root patterning gene 1
