摘要
目的:应用寡核苷酸芯片研究正常肝脏、慢性乙型肝炎、肝硬化及肝癌的基因表达谱,筛选肝癌相关基因.方法:分别对正常肝组织及肝炎、肝硬化和肝癌组织进行总RNA抽提并纯化,反转录得到cDNA,生物素标记cRNA探针,分别与含有19378个已知基因的寡核苷酸芯片进行杂交,Gene Scanner 3000激光系统扫描,GenePix Pro3.0分析软件读取处理杂交信号.结果:在慢性乙型肝炎、肝硬化及肝癌组织中,筛选出共同差异表达基因81个,其中持续上调表达基因53个,持续下调表达基因数28个.结论:寡核苷酸基因表达谱芯片能够快速筛选出肝癌相关基因,有多种基因共同参与肝癌发生的整个过程.
AIM： To investigate the gene expression profiles in tissues of normal human liver, chronic hepatitis B, cirrhosis and hepatocellular carcinoma （HCC） by oligonucletide chip, and to screen HCC-related genes. METHODS： The total RNA was extracted and reverse transcribed into double-strand cDNA, and then transcribed into biotin-labled cRNA target probes. The probes were hybridized with oligonucleotide chip containing 19378 genes respectively. Subsequently, the signal images were scanned by gene Scanner 3000 and analyzed with GenePix Pro 3.0 software. RESULTS： Eighty-one genes differentially expressed among tissues of chronic hepatitis B, cirrhosis and HCC were screened out in the gene expression profiles. Of the 81 genes, 53 genes were consistently up-regulated, while 28 genes were consistently down-regulated CONCLUSION： The oligonucleotide chip of gene expression profile is powerful for screening HCC-related genes. Our results indicate that multiple genes take part in the carcinogenesis of HCC.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第22期2537-2541,共5页
World Chinese Journal of Digestology
基金
河南省医学科技创新基金资助项目
No.20050080~~
关键词
寡核苷酸芯片
基因表达谱
肝细胞癌
Oligonucleotide chip
Gene expression profile
Hepatocellular carcinoma
