摘要
目的：探讨参芎注射液对大鼠脑缺血再灌注后神经细胞凋亡及Fas相关死亡结构域蛋白（FADD）及其mRNA表达的影响。方法：100只SD大鼠随机分为正常组（n=10）、假手术组（n=10）、脑缺血再灌注组（n=40）和参芎注射液组（n40）。线栓法制备大脑中动脉闭塞模型，3X3NEL法检测神经细胞凋亡，分别应用免疫组织化学染色和逆转录一聚合酶链式反应检测FADD蛋白和mRNA表达。结果：与正常组和假手术组相比，脑缺血再灌注组凋亡神经细胞计数显著增加（P〈0．01），FADD蛋白和mRNA表达均显著增强（P均〈0．01）。与脑缺血再灌注组比较，参芎注射液组凋亡神经细胞显著减少（P〈0．05，P〈0．01），FADD蛋白和mRNA表达显著减弱（P〈0．05，P〈0．01）。结论：参芎注射液能通过下调FADD表达抑制大鼠脑缺血再灌注后神经细胞凋亡。
Objective： To investigate the effect of Shenxiong injection on neuronal apoptosis and Fasassociated death domain protein （FADD） and its mRNA expression after ischemia-reperfusion in rats. Methods： A total of 100 SD rats ,acre randomly allocated into normal （n = 10）, sham-operation （n = 10）, cerebral ischemia-reperfusion （n =40）, and Shenxiong injection （n =40） groups. A model of middle cerebral eatery occlusion was induced by suture method. The neuronal apoptosis was detected by the TUNEL assay. The expressions of FADD protein and mRNA ,were detected by immunohisto-chemical staining and by reverse transcription-polymerase chain reaction （RT-PCR）, respectively. Results： As compared with the normal and sham-operation groups, the numbers of apoptotic cell in the cerebral ischemia-reperfusion group ,Were increased significantly （P 〈0. 01）, and the expressions of FADD protein and mRNA ,were enhanced significantly （all P 〈 0. 01 ）. As compared with the cerebral ischemia-reperfusion group, the numbers of apoptotic cell ,were decreased significantly （P 〈0. 05, P 〈0. 001）, and the expressions of FADD protein and mRNA ,were reduced significantly in the Shenxiong group （P 〈0. 05, P 〈0. 001）. Conclusion： Shenxiong injection may inhibit the neuronal apoptosis after ischemia-reperfusion in rats by down-regulating the expression of FADD.
出处
《国际脑血管病杂志》
2008年第7期507-510,共4页
International Journal of Cerebrovascular Diseases
关键词
参芎注射液
脑缺血
再灌注损伤
凋亡
FAS相关死亡结构域蛋白
Shenxiong injection
cerebral ischemia
reperfusion injury
apoptosis
Fas-associated death domain protein
