摘要
采用半定量RT-PCR技术对甜菜胞液型谷氨酰胺合成酶(GS1)和质体型谷氨酰胺合成酶(GS2)基因进行mRNA的表达检测,同时进行谷氨酰胺合成酶活性的测定,研究不同氮素处理对谷氨酰胺合成酶(GS)基因表达的调控,并以三磷酸甘油醛脱氢酶(GAPDH)为内参照进行基因表达水平的半定量分析。结果显示,NO3-:NH4+>1和NO3-:NH4+=50:50较NO3-:NH4+<1更能促进GS活性;NO3-:NH4+=80:20和NO3-:NH4+=50:50较NO3-:NH4+<1更能促进GS1基因的表达;NO3-:NH4+>1和NO3-:NH4+=50:50较NO3-:NH4+<1更能促进GS2基因的表达。说明硝态氮比例较高的混合态氮较铵态氮比例较高的混合态氮及单一铵态氮更能促进GS活性和GS基因的表达,氮素能有效地在转录水平上调控甜菜幼苗叶片GS基因的表达。
A laboratory experiment was conducted to investigate the expression of cytosolic glutamine synthetase （ GS1 ） and plastidic glutamine synthetase （ GS2 ） by using semi-quantitative PCR and analyzing activities of GS in sugar beet under different treatments of nitrogen. The efficiency of GS mRNA synthesis from each sample was estimated by quantitative PCR of glyceraldehyde-3-phosphate dehydrogenase （ GAPDH ）. The results showed that the GS activities and the expression of GS2 gene in sugar beet leaves were enhanced when the rates of NO3^-：NH4^＋ were higher than 1 or amounted to 50： 50. The expression of GS1 gene increased when the rates of NO3^-：NH4^＋ were 50：50 or 80：20. Therefore ,the activities and the expression of GS gene in sugar beet were regulated by nitrogen at transcriptional level.
出处
《作物杂志》
CAS
CSCD
北大核心
2008年第4期64-67,共4页
Crops
基金
国家自然基金(30471017
30771276)
关键词
甜菜
谷氨酰胺合成酶
氮素
基因表达
Sugar beet （ Beta vulgaris L. ）
Glutamine synthetase （GS）
Nitrogen
Gene expression
