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利用Ames法检测烧烤肉类食品致突变性的研究

Studies on Optimum Nutritional Conditions of Mycelia of Cordyceps Militaris Link in Liquid Deep Fermentation
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摘要 选取烧烤肉类食品样品,用环己烷提取,经色-质联用仪对样品提取物进行定性定量检测后,再以Ames试验进行致突变性检测.结果提取物中多种物质对TA97和TA100呈阳性反应,具有剂量关系.说明烧烤肉中除去苯并芘类已公认具有致癌作用外,另外,还存在其他致突变物,如氨基酸热解物等. Throrgh single factor and orthogonal design method the shaking culture experiment results show that the optimum conditions in liquid culture are: glucose2%, sugar1%, yeast extract 1%, peptone 1%, NH 4Cl0.2%,MgSO 4·7H 2O0.05%, KCl0.1%. The fermentator of 100L culture experiment results show that carbon and nitrogen source are 3% sugar and 1.5% peptone seperatively.
机构地区 辽宁大学生物系
出处 《辽宁大学学报(自然科学版)》 CAS 1997年第3期31-33,共3页 Journal of Liaoning University:Natural Sciences Edition
关键词 烧烤肉食品 致突变性 癌症 Ames法 Cordycers militaris Link, Iiquid deep fermentution Mycelium.
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