摘要
目的观察电针对糖尿病性认知功能障碍大鼠学习记忆的改善作用,及其对海马神经营养因子-3(NT-3)mRNA和免疫反应阳性细胞表达的影响。方法采用2%链脲佐菌素(streptozotocin,STZ)构建糖尿病大鼠模型,将模型大鼠随机分为电针组(EA)、对照组(DM)与正常组(CN)进行比较。电针4周后糖尿病测定血糖水平,并用Morris水迷宫法观察电针对糖尿病大鼠学习记忆的影响,应用RT-PCR和免疫组化方法检测海马NT-3mRNA和免疫反应阳性细胞的表达水平。结果对照组血糖、上台潜伏期高于正常组和电针组(P<0.01),对照组NT-3 mRNA和免疫反应阳性细胞的表达明显低于正常组和电针组(P<0.01);电针组NT-3表达明显高于对照组(P<0.01)。结论电针能在一定程度上降低血糖,促进海马NT-3 mRNA和免疫反应阳性细胞的表达,改善糖尿病认知功能障碍者的学习和认知功能。
Objective To observe the improvement of learning and memory ability and the expression of neurotrophin-3 （NT-3） mRNA in the hippocampus induced by electroacupuncture in diabetic rats with cognitive impairment. Methods The diabetic rats were generated by injecting 2% streptozotocin （STZ）. The rats were randomly divided into three groups： electroacupuncture group （EA）, diabetes mellitus-untreated group （DM）, and the normal control group （CN）. After 4 weeks, blood sugar levels in diabetic rats were determined and the influence on learning and memory was observed with the using of Morris water maze. At the same time the expression of NT-3 mRNA in the hippocampus and the positively expressing cells were detected by RT- PCR and immunohistochemistry. Results Blood sugar levels and the latency period going up onto the platform in rats in DM group were higher than those in rats of EA and in CN groups （ P 〈 0.01 ）. However, the expression of NT-3 mRNA in the hippoeampus and the positively expressing cells in DM group were lower than those in rata of EA and CN groups （ P 〈 0.01 ）. Concluslon The blood glucose level in diabedc rats can be decreased by electroacupuncture. The expression of NT-3 mRNik in the hippocampus and the positively expressing cells in diabetic rats can be increased. Thus, learning and memory abilities indiabetic rats with cognitive impairment can be improved.
出处
《中国比较医学杂志》
CAS
2008年第8期14-17,I0004,共5页
Chinese Journal of Comparative Medicine
基金
黑龙江省教育厅课题项目(编号11513055)
关键词
电针
血糖
学习记忆
海马
神经营养因子-3
Electroacupuncture
blood glucose
Diabetic cognitive dysfunction
Learning ability
Memory ability
Hippocampus
Neurotrophin-3（NT-3）
Rat
