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2007年乙型流感病毒抗原性和基因特性分析 被引量:2

Analysis of Antigenic and Genetic Characteristics of Influenza Virus B Circulated in Sichuan Province in 2007
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摘要 目的了解四川省乙型流感病毒血凝素的抗原性和基因变异情况。方法采集四川省流感监测哨点医院和疑似流感疫情的流感样病例咽拭子,用MDCK细胞进行病毒分离。选取来自不同时间、地点且经血凝抑制实验鉴定为乙型的流感病毒分离株进行单向血凝抑制实验;提取病毒核酸,进行RT-PCR扩增HA基因的HA1区,对扩增产物进行序列测定并推导出氨基酸序列。结果2007年四川省乙型Yamagata系流感病毒的抗原性不同于B/天津/144/2005,而与B/福建新罗/54/2006类似,乙型Victoria系病毒与B/深圳/155/2005相比其抗原性存在差异;核苷酸和氨基酸序列分析结果表明,在HA1区有分别有6个、2个和4个氨基酸位点发生了替换。结论四川省2007年乙型Yamagata系和Victoria系流感病毒的抗原性与B/天津/144/2005和B/深圳/155/2005相比发生了进一步的漂移。 Objective To study the variation of antigenicity and genetic characterization about hemagglutinin of influenza virus subtype B circulated in Sichuan Province. Methods The swallow swabs from the suspected influenza eases in sentinel hospital monitored and suspected epidemic influenza in Sichuan were collected, and virus were isolated by using MDCK cells. The influenza viruses isolated in different time and locations were identified to be influenza virus subtype B by magglutination inhibition test. The viral nuc RT-PCR, and the amplified products were hemagglutination inhibition test, then were performed one-way heleic acid were extracted to amplify the HAI regions of HA by using sequenced to deduct the amino acid sequence. Results The antigenecity of influenza virus subtype B Yamagata series in Sichuan was differrent from that of the stain B/Tianjin//144/2005 but analogic to that of the strain B/Fujianxinluo/54/2006. Compared with influenza virus B/ Shenzhen/155/2005, the antigenecity of subtype B victoria sereis in Sichuan has changed. The analytic results of nucleotide and amino acid sequence demonstrated there were 6, 4 and 2 amino acids sites replaced respectively in HAl regions. Conclusion In comparison with the strains B/Tianjin//144/2005 and B/Shenzhen/155/2005, the antigenicity of influenza virus subtype B Yamagata series and Victoria series circulated in Sichuan in 2007 has drifted furtherly
出处 《预防医学情报杂志》 CAS 2008年第10期772-774,共3页 Journal of Preventive Medicine Information
基金 四川省卫生厅科研课题(编号070110)
关键词 流感病毒乙型 血凝素基因 抗原性 基因特性 Influenza virus B Hemagglutinin Antigenicity Genetic characterization
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