摘要
目的分析和确定磷蛋白磷酸酶2A型(PP2Ac)突变型肺癌相关基因在人肺癌细胞GLC-82和正常人淋巴细胞染色体区域的定位。方法制作人肺腺癌细胞GLC-82和正常人淋巴细胞的染色体中期分裂相,用生物素标记所克隆到的PP2Ac突变基因cDNA序列(长度约为1.2 kb),以此作为探针进行染色体荧光原位杂交(FISH)。荧光显微镜下观察记录和分析杂交信号的特征。结果在正常人淋巴细胞的染色体5q23-31可见明显杂交信号,在GLC-82细胞的5号和7号染色体上出现较强信号。结论点突变引起PP2A活性改变,从而基因易位,导致肺肿瘤的产生。
Objective To explore the chromosome destination of the point mutational lung cancer-associated gene on lymphocytes and GLC-82 cells. Methods The metaphase slides of lymphocytes and GLC-82 cells were prepared, T the gene as eDNA probe （ approximately 1.2 kb） biotinylated with dUTP was used, to perform the fluroseenee in situ hybridization. The FISH signals were recorded and analyzed to make sure the ralationships between PP2A and the gene that our laboratory had got. Results Strongly signals could be seen in the lymphocytes chromosome 5q23-31, and also signals could be seen in the GLC-82 cells chromosome 5 and 7. Conclusion The point mutation can change the activity of PP2Ae, and lead to translocation, it may be selated to lung cancer.
出处
《山东医药》
CAS
北大核心
2008年第27期9-11,共3页
Shandong Medical Journal
基金
国家十五科技攻关项目(2001BA703B11)
关键词
点突变
肺癌相关基因
原位杂交
荧光
染色体
point mutation
human lung cancer-associated gene
in situ hybridization, fluroscenee
chromosome
