摘要
目的研究自介素17（interleukin-17，IL-17）抗体在吸烟所致慢性阻塞性肺疾病（chronicobstructivepulmonarydisease，COPD）模型小鼠气道炎症中的作用。方法C57／BL6雄性小鼠随机分为COPD组（8只）、COPD＋IL-17抗体干预组（简称COPD＋干预组，8只）和正常对照组（10只）。对支气管肺泡灌洗液（BALF）进行细胞计数、染色和分类；用酶联免疫吸附试验检测小鼠肺组织匀浆IL-17水平，观察各组小鼠气道病理改变。结果COPD组与COPD＋干预组相比肺功能差异无统计学意义。COPD组、COPD＋干预组小鼠与正常对照组相比BALF细胞总数显著增高[分别为（19．64±1．89）×10^4／ml，（15．47±2．99）×10^4／ml和（5．13±1．00）×10^4／ml，P〈0．01]；COPD＋干预组较COPD组细胞总数下降（P〈0．01）；中性粒细胞比例[分别为（8．58±6．77）％，（22．98±8．46）％]及绝对值[（1．28±0．96）×10^4／ml，（4．53±1．73）×10^4／ml]显著下降（P值均〈O．01）。肺组织HE染色病理评分COPD＋干预组（73．25±18．58）较COPD组（106．13±36．27）炎症有所减轻（P〈O．05）。COPD＋干预组小鼠肺组织匀浆中IL-17含量（O．084±0．041）pg／mgpro与COPD组（0．221±0．081）pg／mgpro相比显著降低（P〈0．01）。结论吸烟所致COPD小鼠模型中IL-17参与了中性粒细胞引起的气道炎症，抑制IL-17的表达，可以减少气道内中性粒细胞的数量，减轻气道炎症。
Objective To investigate the significance of interleukin-17（IL-17） antibodies in cigarette smoke-induced chronic obstructive pulmonary disease（COPD） in mice. Methods Male C57/BL6 mice were randomly divided into COPD group（ n =8） ,COPD ＋anti-IL-17 Ab group（ n =8） ,and normal control group （ n = 10）. The number of cells in bronchoalveolar lavage fluid（BALF） was calculated and the concentrations of IL-17 in lung tissues was measured by enzyme-linked immunosorbent assay. Histologic analysis of the lungs was done in each group. Results The intra-airway pressure（IP）, peak expiratory flow（PEF）,and Ip slope in anti-IL-17 Ab group were insignificant compared with those of COPD group. The total cell count in BALF was significantly higher in COPD group than in normal control group（ P 〈0.01）. Compared with COPD group,the total cell count in COPD＋ anti-IL-17 Ab group was decreased[（19.64±1.89） ）×10^4/ml and （ 15.47± 2.99）×10^4/ml （ P 〈 0.01 ）], neutrophils in BALF were obviously decreased, （22.98 ± 8.46）%, （8.58±6.77） %（ P 〈0. 01）. The airway inflammation was alleviated in COPD＋anti-IL-17 Ab group by histologic analysis. The concentration of IL-17 in lung tissue was significantly lower in COPD＋anti-IL-17 Ab group E（0. 084±0. 041） pg/mg pro-] than in the COPD group [（0. 221±0. 081） pg/mg pro]（ P 〈0.01）. Conclusions IL-17 might play a role in airway neutrophilic inflammation in cigarette smoke-induced COPD in mice. Anti-IL-17 could decrease the number of neutrophils in the BALF and attenuate the neutrophilic airway inflammation.
出处
《国际呼吸杂志》
2008年第16期981-984,F0003,共5页
International Journal of Respiration
基金
国家自然科学基金资助项目（30370608）
关键词
慢性阻塞性肺疾病
疾病模型
自介素17抗体
气道炎症
Chronic obstructive pulmonary disease
Disease models
Interleukin-17 antibody
Airway inflammation
