摘要
目的探讨中性粒细胞相关载脂蛋白（NGAL）在乳腺癌的表达及其临床意义。方法乳腺癌手术切除标本47例，应用逆转录-聚合酶链反应（RT-PCR）检测NGAL mRNA相对表达量；应用免疫组织化学方法，分析NGAL蛋白表达情况。结果乳腺癌组织中NGAL在乳腺癌组织中NGAL mRNA的表达水平为0．42±0．19，显著高于癌旁组织（P〈0．01）；NGAL蛋白在癌组织大多表达为＋＋～＋＋＋水平，癌旁正常组织则大多表达在-～＋水平，NGAL蛋白在癌组织表达显著高于癌旁正常组织（P〈0．01）。NGAL mRNA和蛋白表达与淋巴结转移、病理类型、ER、HER-2状态明显相关（P〈0．05），而与患者年龄、肿瘤大小、组织学分级、TNM分期、PR状态无明显相关（P〉0．05）。结论NGAL的表达与乳腺癌淋巴结转移和内分泌治疗耐药等相关，NGAL可能是乳腺癌早期诊断、监测复发和判断预后的潜在生物学参考指标。
Objective To explore the neutrophil gelatinase-associated lipocalin （NGAL） expression in breast cancer and its clinical significance. Methods Forty-seven cases of human breast cancer specimens were collected from the patients who received operation for breast cancer. Semi-quantitative RT- PCR and immunohistochemistry were used to detect the expression of NGAL mRNA and protein expression respectively. Results The results of semi-quantitative RT-PCR analysis showed that the level of NGAL mRNA expression in primary breast carcinoma specimens was 0.42±0.19, significantly higher than in the paracancerous tissues （P 〈0.01 ） ;Immunohistochemistry staining revealed that the degree of NGAL protein expression in primary breast cancer tissues （ average ＋＋ - ＋＋＋ ） was significantly higher than in the paracancerous tissues （ average - - ＋ ,P 〈 0.01 ）. The high-expression of NGAL mRNA and protein was related to lymph node metastases,pathological type,ER and HER-2 status （ P 〈 0.05 ）. However, there was no significant association with age, tumor size, histologic grade, TNM stage and PR status （ P 〉 0.05 ）. Conclusion The expression of NGAL was closely correlate with lymph node metastases and resistance of endocrine therapy, suggesting that NGAL might be a potential biomarker for the early diagnosis, monitoring recurrence and predication of prognosis of human primary breast cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第8期1018-1020,1091,共4页
Chinese Journal of Experimental Surgery
基金
浙江省自然科学基金资助项目（Y207301）
