摘要
以转抗虫基因(BtCry3A)741杨7个株系和转双抗虫基因(BtCrylAc+API)741杨2个株系为材料,从分子生物学检测、毒蛋白的表达、抗虫性检测3个方面,研究转不同Bt基因741杨对不同类型害虫的抗虫选择性。结果表明:2种Bt抗虫基因分别在转基因741杨不同株系中稳定存在。转BtCry1Ac的2个株系毒蛋白含量分别为0.0127%和0.0099%,具高抗虫性的株系pb29的毒蛋白含量高于中等抗虫性的株系pb17。转BtCry3A基因的5个株系毒蛋白含量为0.0678%～0.1521%,不同株系间存在一定差异。BtCry3型的毒蛋白表达量非常高,是BtCry1型的10倍。用对鳞翅目害虫具高抗虫性和中等抗虫性的转双抗虫基因(BtCrylAc+API)741杨株系叶片饲养鞘翅目害虫柳蓝叶甲,幼虫死亡率达到28.4%～42.8%,转基因株系与未转基因的对照没有明显差异。用转BtCry3A基因741杨不同株系叶片饲养柳兰叶甲幼虫,均表现出高抗虫性,死亡率在2天之内均可达到100%;而饲养杨扇舟蛾幼虫,死亡率为0～42.86%,未表现出明显抗虫性,与未转基因对照没有明显差异。
We studied the insect-resistance selectivity of transgenic hybrid poplar 741 that carried the different Bt genes to different type pest by the molecule detection, insect-resistant test and insectical protein expression. The seven transgenic subclones of transgenic hybrid poplar 741 carrying the BtCry3A gene and two transgenic subclones of transgenic hybrid poplar 741 carrying two insect-resistant genes （BtCrylAc ＋ API） were used. Result showed that two BT genes respectively and steadily existed in different transgenic hybrid poplar 741. The contents of toxic protein in two individual plants carrying insect-resistant genes （BtCrylAc） were respectively 0.012 7% and 0.009 9% . The content of toxic protein in plant pb29 was higher than in plant pbl7 and the former was also more resistant to the pests than the latter. The content of toxic protein in five individual plants carrying insect-resistant genes （BtCry3A） was between 0.067 8% and 0. 152 1%, and there was variation in the protein content among the individual plants. The insectical protein expression content of BtCry3 was very high and was 10 times higher than that of BtCryl. The laminae of transgenic hybrid poplar 741 carrying two insect-resistant genes （ BtCrylAc ＋ API） were used to feed Lepidoptera pests of Plagiodera versicolora （Laicharting）, the larval mortality was up to between 28.4% and 42.8%, and it has not obviously differentia between transgenic subclones and in contrast. The laminaes of different transgenic subclones carrying the BtCry3A gene were feed to the larva of Plagiodera versicolora （Laicharting）, it put up a high insect-resistantce, the larval mortality was up to 100% promptly within 1 - 2 days; The larval mortality was between 0 and 42.86% when the larva of Clostera anachoreta （Fabricius） were feed. It has not obvious insect-resistantce and it has little differentia than in contrast.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2008年第8期67-71,共5页
Scientia Silvae Sinicae
关键词
转抗虫基因741杨
外源基因
BT毒蛋白
抗虫选择性
transgenic insect-resistance hybrid poplar 741
foreign gene
Bt toxic protein
insect-resistance
