摘要
以一株黄假单胞属(Pseudomonas flava)菌株LJW-01为出发菌株,经过亚硝基胍(NTG)诱变处理,选育出一株蛋氨酸营养缺陷型(Met-)菌株ZC8。研究发现,发酵33 h后向发酵液中添加1 g/L的Mg3(PO4)2,菌株的L-丝氨酸产量明显提高,并且该缺陷型菌株在35 g/L甘氨酸的条件下,发酵3天后L-丝氨酸产量为7.5 g/L,较出发菌株提高了50%。该菌具有较好的传代稳定性。
A L-serine producing methionine auxotroplx mutant （Met-） was derived from Pseudomonas flava LJW-01 by treatment with NTG. It could accumulate 7.5 g/L L-serine when it was cultured for 3 days by adding 1g/L Mg3（PO4）2, and it had an improvement of about 50% compared with the original strain. The mutant also had good stability of descendiblity of L-serine producing.
出处
《化工进展》
EI
CAS
CSCD
北大核心
2008年第9期1408-1411,共4页
Chemical Industry and Engineering Progress
