摘要
[目的]初步探讨气管滴注纳米氧化锌(N-ZnO)的急性毒性。[方法]50只雄性ICR小鼠随机分为5组,高、中、低剂量组(HG、MG、LG)用N-ZnO悬浮液进行单次气管滴注染毒,剂量分别为每只20、10、5μg。空白对照组(BG)不作处理,阴性对照组(NG)采用磷酸缓冲盐溶液(PBS)气管滴注。染毒后第7天对小鼠支气管肺泡灌洗液(BALF)、血常规以及主要脏器病理进行分析。[结果]观察终点HG小鼠仅存1只,故未纳入统计。HG和MG一般情况差,染毒后1、3、5天体重降幅高于BG及NG(P<0.05);MG、LG的BALF中总蛋白、羟脯氨酸、碱性磷酸酶(AKP)活性,全血红细胞平均容量(MCV)、红细胞平均血红蛋白量(MCH)、红细胞体积分布宽度(RDW)、血小板平均体积(MPV)高于BG及NG (P<0.05);而全血红细胞计数(RBC)低于BG及NG(P<0.05)。MG单核细胞百分比(MOD%)高于BG、NG及LG,红细胞平均血红蛋白浓度(MCHC)低于BG及NG(P<0.05);同时,染毒后3天和5天的体重降幅、RDW、MPV、MOD%及肺灌洗液AKP在染毒组问也存在差异(P<0.05)。病理发现各染毒组小鼠肺内有明显的炎症反应,肺泡壁细胞增生和肺泡壁增厚现象,且随染毒剂量增加而加重。[结论]气管滴注N-ZnO诱发了比较明显的肺部炎症,并引起小鼠体重明显降低并发贫血。
[ Objective ] To make a preliminary assessment on the acute toxicity of intratracheally instilled mmo-sized zinc oxide（ N-ZnO ）in ICR mice. [ Methods ] 50 healthy male adult ICR mice were randomly grouped in 5 by their body weight. 20 μg（ HG ）, 10 μg（ MG ）, 5 μg（ LG ）of N-ZnO were intratracheally instilled into lungs of each mouse as test groups and PBS was instilled as negative control（ NG ）. Another group without any treatment was used as blank control（ BG ）. The BALF and blood were collected for analysis on the 7th day after treatment. Histopathologic evaluation of the main organs were also performed. [ Results ] There were only one mouse left in HG on 7th day postinstillation, so this group was not included for statistical analysis. The healthy conditions of mice from HG and MG were generally poor, loss of body weight were significantly higher than that of BG and NG from the 1st day to the 5th day（P 〈 0.05 ）; RBC of MG or LG was lower than that of BG and NG while content of total protein, hydroxyproline and activity of AKP in BALF, MCV, MCH, RDW, MPV of blood were higher than those of BG and NG（ P 〈 0,05 ）. MOD% of MG was also higher than that of BG, NG and LG, but MCHC was lower （ P 〈 0.05 ）.While the differences of body weight loss, activity of AKP in BALF and RDW, MPV, MOD% on 3rd and 5th day were also observed among the treated groups. Histopathological observation showed that serious pulmonary inflammation, proliferation and thickening of alveolar walls existed in the lungs of all treated mice groups, which became more serious as dose increased. [ Conclusion ] Intratracheal instillation of N-ZnO induced significant pulmonary inflammation and marked body weight loss accompanied with anemia.
出处
《环境与职业医学》
CAS
北大核心
2008年第4期360-364,共5页
Journal of Environmental and Occupational Medicine
基金
国家自然科学基金重大项目(编号:10490180)
国家"973"计划资助项目(编号:2006CB705600)
关键词
纳米氧化锌
小鼠
气管滴注
支气管肺泡灌洗
nano-sized zinc oxide
mice
intratracheal instillation
bronchoalveolar lavage
